The effect of breed, boar and season on some properties of sperm

Abstract

The main objective of this study was to assess the phenotypic variability of the ejaculate volume (VOL) and progressive sperm motility (MO) under the influence of breed, boar within breed and season. The study included 555 ejaculates of Danish Landrace (DL), Danish Large White (DLW) and Danish Duroc (DD) boars. The effect of boar was analyzed within the investigated breeds. The impact of the season was investigated as the influence of the month in which the ejaculate was taken. The model included a linear regression effect of boar age when the semen was taken. Data analysis was performed using the statistical package SAS 9.1.3 (SAS Inst. Inc.., 2002-2003). Impact assessment was carried out using the GLM procedure. The impact of all factors analyzed in both sperm traits was significant to highly significant, and the observed factors largely explain the phenotypic variability VOL compared to MO. The highest values were found in VOL of DL boar (3:58 ± 164.96 ml), and the MO of boar breed DLW (85.45 ± 0.94%). Boars DD had VOL and MO below the averages of all analyzed breeds. Between boars within studied genotypes differences were established (p (lt) 0.05, p (lt) 0.05, p (lt) 0.01 p (lt) 0.001) and a boar of DLW breed compared to all tested boars had the highest volume of ejaculate sperm with progressive motility of 90.47%. Volume, or mobility varied (p (lt) 0.01 or p (lt) 0.05) influenced by season, and in September and October boars had ejaculates with the VOL values above average and the lowest MO. Since most VOL and MO above the annual average in June, it was possible to get the highest number of doses per ejaculate with optimal reproductive/fertile ability.

Osnovni cilj rada bio je da se oceni fenotipska varijabilnost volumena ejakulata (VOL) i progresivne pokretljivosti spermatozoida (MO) pod uticajem rase, nerasta unutar rase i sezone. Istraživanjem je bilo obuhvaćeno 555 ejakulata nerasta rase danski landras (DL), danski jorkšir (DLW) i danski durok (DD). Uticaj nerasta analiziran je unutar ispitivanih rasa. Uticaj sezone ispitivan je kao uticaj meseca u kojem je uzet ejakulat. U model je uključen i linearni regresijski uticaj starosti nerasta prilikom uzimanja ejakulata. Obrada podataka izvršena je pomoću statističkog paketa SAS 9.1.3 (SAS Inst. Inc., 2002-2003). Ocena uticaja je izvršena primenom GLM procedure. Uticaj svih analiziranih faktora na obe osobine sperme bio je značajan do vrlo visoko značajan, a posmatrani faktori u većoj meri objašnjavaju fenotipsku varijabilnost VOL u odnosu na MO. Najveće vrednosti VOL utvrđene su kod nerasta rase DL (164.96±3.58 ml), a MO kod nerasta rase DLW (85.45±0.94%). Nerasti DD imali su VOL i MO ispod proseka svih analiziranih rasa. Između nerasta unutar ispitivanih genotipova utvrđene su razlike (p>0.05, p (lt) 0.05, p (lt) 0.01, p (lt) 0.001), a nerast rase DLW u odnosu na sve ispitivane neraste imao je najveći volumen ejakulata sa progresivnom pokretljivošću spermatozoida od 90.47%. Volumen, odnosno pokretljivost su varirali (p (lt) 0.01, odnosno p (lt) 0.05) pod uticajem sezone, a u periodu septembar-oktobar nerasti su imali ejakulate sa vrednostima VOL iznad proseka i najnižom MO. S obzirom na najveći VOL i MO iznad godišnjeg proseka, u junu mesecu je bilo moguće dobiti najveći broj doza po ejakulatu sa optimalnom fertilnom sposobnošću.