TY  - JOUR
AU  - Cekić, Bogdan
AU  - Kanzova, Hristiyana
AU  - Petrov, Georgi
AU  - Maksimović, Nevena
AU  - Ćosić, Ivan
AU  - Milovanović, Aleksandar
PY  - 2022
UR  - http://r.istocar.bg.ac.rs/handle/123456789/797
AB  - The current study aimed to examine the percentage changes of
viability sperm and the activity of the enzymes LDH, ALP, GOT / AST, GPT /
ALT in the sperm plasma of Lacaune rams, before and after cryopreservation. For
this purpose, five rams were examined, and two ejaculates were obtained from each
ram. Ejaculates are collected by the method of artificial vagina, during the
insemination campaign. All ejaculates were diluted with a 6AG extender and
frozen by the Cassou’s sequin method. Sperm viability was determined by eosin
and nigrosine smears, and enzyme activity was examined spectrophotometrically.
As a result, the percentage of vital sperm after cryopreservation decreased by 15%
(P ≤ 0.001). The freezing and thawing process also reduced the activity of the
enzymes LDH, ALP, GOT / AST and GPT / ALP. In conclusion, the observed
enzymes, in relation to sperm vitality, could be used as indicators to optimize the
protocols for cryopreservation of ram’s sperm.
AB  - Istraživanje je imalo za cilj ispitivanje promena u procentu vijabilnih
spermatozoida i promena u aktivnosti enzima LDH, ALP, GOT / AST, GPT / ALT
u seminalnoj plazmi ovnova rase Lakon, pre i nakon krioprezervacije. Za tu svrhu,
ispitivana je sperma pet ovnova, sa dva ejakulata po ovnu. Ejakulati su dobijeni
metodom veštačke vagine, u toku sezone parenja. Svi ejakulati su razređeni
razređivačem 6AG i zamrznuti po metodi Cassou sequin. Vijabilnost
spermatozoida je određena na osnovu razmaza sa eozinom i nigrozinom, a
aktivnost enzima je određena spektrofotometrijski. Rezultati istraživanja pokazuju
da je udeo vitalnih spermatozoida nakon krioprezervacije smanjen za 15% (P ≤
0.001). Procesi zamrzavanja i odmrzavanja takođe smanjuju i aktivnost enzima
LDH, ALP, GOT/ AST i GPT / ALP. Ispitivani enzimi, združeno sa vitalnošću
spermatozoida, mogu da se koriste kao markeri za optimizaciju stope hlađenja i
koraka u procesima zamrzavanja i odmrzavanja sperme ovnova.
PB  - Institute for Animal Husbandry, Belgrade-Zemun, Serbia
T2  - Biotechnology in animal husbandry
T1  - Analysis of vitality and biochemical parameters in freeze-thawed seminal plasma of rams
VL  - 38
IS  - 1
SP  - 31
EP  - 39
DO  - 10.2298/BAH2201031C
ER  - 

@article{
author = "Cekić, Bogdan and Kanzova, Hristiyana and Petrov, Georgi and Maksimović, Nevena and Ćosić, Ivan and Milovanović, Aleksandar",
year = "2022",
abstract = "The current study aimed to examine the percentage changes of
viability sperm and the activity of the enzymes LDH, ALP, GOT / AST, GPT /
ALT in the sperm plasma of Lacaune rams, before and after cryopreservation. For
this purpose, five rams were examined, and two ejaculates were obtained from each
ram. Ejaculates are collected by the method of artificial vagina, during the
insemination campaign. All ejaculates were diluted with a 6AG extender and
frozen by the Cassou’s sequin method. Sperm viability was determined by eosin
and nigrosine smears, and enzyme activity was examined spectrophotometrically.
As a result, the percentage of vital sperm after cryopreservation decreased by 15%
(P ≤ 0.001). The freezing and thawing process also reduced the activity of the
enzymes LDH, ALP, GOT / AST and GPT / ALP. In conclusion, the observed
enzymes, in relation to sperm vitality, could be used as indicators to optimize the
protocols for cryopreservation of ram’s sperm., Istraživanje je imalo za cilj ispitivanje promena u procentu vijabilnih
spermatozoida i promena u aktivnosti enzima LDH, ALP, GOT / AST, GPT / ALT
u seminalnoj plazmi ovnova rase Lakon, pre i nakon krioprezervacije. Za tu svrhu,
ispitivana je sperma pet ovnova, sa dva ejakulata po ovnu. Ejakulati su dobijeni
metodom veštačke vagine, u toku sezone parenja. Svi ejakulati su razređeni
razređivačem 6AG i zamrznuti po metodi Cassou sequin. Vijabilnost
spermatozoida je određena na osnovu razmaza sa eozinom i nigrozinom, a
aktivnost enzima je određena spektrofotometrijski. Rezultati istraživanja pokazuju
da je udeo vitalnih spermatozoida nakon krioprezervacije smanjen za 15% (P ≤
0.001). Procesi zamrzavanja i odmrzavanja takođe smanjuju i aktivnost enzima
LDH, ALP, GOT/ AST i GPT / ALP. Ispitivani enzimi, združeno sa vitalnošću
spermatozoida, mogu da se koriste kao markeri za optimizaciju stope hlađenja i
koraka u procesima zamrzavanja i odmrzavanja sperme ovnova.",
publisher = "Institute for Animal Husbandry, Belgrade-Zemun, Serbia",
journal = "Biotechnology in animal husbandry",
title = "Analysis of vitality and biochemical parameters in freeze-thawed seminal plasma of rams",
volume = "38",
number = "1",
pages = "31-39",
doi = "10.2298/BAH2201031C"
}

Cekić, B., Kanzova, H., Petrov, G., Maksimović, N., Ćosić, I.,& Milovanović, A.. (2022). Analysis of vitality and biochemical parameters in freeze-thawed seminal plasma of rams. in Biotechnology in animal husbandry
Institute for Animal Husbandry, Belgrade-Zemun, Serbia., 38(1), 31-39.
https://doi.org/10.2298/BAH2201031C

Cekić B, Kanzova H, Petrov G, Maksimović N, Ćosić I, Milovanović A. Analysis of vitality and biochemical parameters in freeze-thawed seminal plasma of rams. in Biotechnology in animal husbandry. 2022;38(1):31-39.
doi:10.2298/BAH2201031C .

Cekić, Bogdan, Kanzova, Hristiyana, Petrov, Georgi, Maksimović, Nevena, Ćosić, Ivan, Milovanović, Aleksandar, "Analysis of vitality and biochemical parameters in freeze-thawed seminal plasma of rams" in Biotechnology in animal husbandry, 38, no. 1 (2022):31-39,
https://doi.org/10.2298/BAH2201031C . .