TY  - JOUR
AU  - Barna, Tomislav
AU  - Apić, Jelena
AU  - Milovanović, Aleksandar
AU  - Maksimović, Nevena
AU  - Mašić, Aleksandar
AU  - Lazarević, Marina
AU  - Pavlović, Miloš
PY  - 2021
UR  - http://r.istocar.bg.ac.rs/handle/123456789/762
AB  - The aim of this study was to analyse semen quality parameters of fresh-extended porcine semen imported from Denmark
through prolonged importing regulations and transport and to analyse fertility performance in sows inseminated four days post semen
collection. Semen was analysed immediately upon the arrival (4 days post collection) using computer-assisted sperm analyses (CASA),
cytomorphology and flow cytometry, and sows were inseminated later the same day. The mean sperm concentration was 1718.09 ±
100.11 × 106, with average of 1193.40 ± 90.58 × 106 motile cells (69%) and 756.04 ± 70.57 × 106 progressive motile spermatozoa (43.58%)
in a dose, which was almost twice lower in value than producer certified-declared number of 2 billion of motile cells. Acrosome and
sperm membrane integrity test indicated high percentage of total damaged acrosome. Mean values of semen samples analysed by
sperm chromatin structure assay (SCSA) showed relatively high degree of chromatin damage. Membrane permeability test showed high
percentage of sperm with damaged membrane. Prolonged transport negatively affected the sperm quality in terms of both motility and
chromatin structure stability. Fertility of sows was affected by semen quality in terms of concentration and sperm motility parameters,
as well as ratio of live sperm cells population with damaged acrosome
PB  - TUBITAK
T2  - Turkish Journal of Veterinary and Animal Sciences
T1  - Quality and fertility of extended boar semen after prolonged transport
VL  - 45
IS  - 5
SP  - 920
EP  - 929
DO  - 10.3906/vet-2012-63
ER  - 

@article{
author = "Barna, Tomislav and Apić, Jelena and Milovanović, Aleksandar and Maksimović, Nevena and Mašić, Aleksandar and Lazarević, Marina and Pavlović, Miloš",
year = "2021",
abstract = "The aim of this study was to analyse semen quality parameters of fresh-extended porcine semen imported from Denmark
through prolonged importing regulations and transport and to analyse fertility performance in sows inseminated four days post semen
collection. Semen was analysed immediately upon the arrival (4 days post collection) using computer-assisted sperm analyses (CASA),
cytomorphology and flow cytometry, and sows were inseminated later the same day. The mean sperm concentration was 1718.09 ±
100.11 × 106, with average of 1193.40 ± 90.58 × 106 motile cells (69%) and 756.04 ± 70.57 × 106 progressive motile spermatozoa (43.58%)
in a dose, which was almost twice lower in value than producer certified-declared number of 2 billion of motile cells. Acrosome and
sperm membrane integrity test indicated high percentage of total damaged acrosome. Mean values of semen samples analysed by
sperm chromatin structure assay (SCSA) showed relatively high degree of chromatin damage. Membrane permeability test showed high
percentage of sperm with damaged membrane. Prolonged transport negatively affected the sperm quality in terms of both motility and
chromatin structure stability. Fertility of sows was affected by semen quality in terms of concentration and sperm motility parameters,
as well as ratio of live sperm cells population with damaged acrosome",
publisher = "TUBITAK",
journal = "Turkish Journal of Veterinary and Animal Sciences",
title = "Quality and fertility of extended boar semen after prolonged transport",
volume = "45",
number = "5",
pages = "920-929",
doi = "10.3906/vet-2012-63"
}

Barna, T., Apić, J., Milovanović, A., Maksimović, N., Mašić, A., Lazarević, M.,& Pavlović, M.. (2021). Quality and fertility of extended boar semen after prolonged transport. in Turkish Journal of Veterinary and Animal Sciences
TUBITAK., 45(5), 920-929.
https://doi.org/10.3906/vet-2012-63

Barna T, Apić J, Milovanović A, Maksimović N, Mašić A, Lazarević M, Pavlović M. Quality and fertility of extended boar semen after prolonged transport. in Turkish Journal of Veterinary and Animal Sciences. 2021;45(5):920-929.
doi:10.3906/vet-2012-63 .

Barna, Tomislav, Apić, Jelena, Milovanović, Aleksandar, Maksimović, Nevena, Mašić, Aleksandar, Lazarević, Marina, Pavlović, Miloš, "Quality and fertility of extended boar semen after prolonged transport" in Turkish Journal of Veterinary and Animal Sciences, 45, no. 5 (2021):920-929,
https://doi.org/10.3906/vet-2012-63 . .

TY  - CONF
AU  - Milovanović, Aleksandar
AU  - Apić, Jelena
AU  - Barna, Tomislav
AU  - Živkov-Baloš, Milica
AU  - Prodanov Radulović, Jasna
AU  - Jakšić, Sandra
AU  - Maksimović, Nevena
PY  - 2020
UR  - http://r.istocar.bg.ac.rs/handle/123456789/710
AB  - This paper describes escalation of sudden drop in semen quality in two boars' farm centers
affected by high mycotoxin level in forage (primarily by deoxynivalenol-DON). Barley and
wheat harvested in 2019 was generally indicated as most risky grains for animal health and
production and main source of DON. DON dominantly affected sperm total and progressive
motility (agglutination and astenospermia) as well as sperm chromatin structure, while acrosome
status and semen morphology were less sensitive to these changes. Bacterial control indicated
increase of bacterial presence. After forage replacement a period of 1-2 months is essential for
recovery of semen production, supported with prolonged vitamins and antibiotic treatment.
Routine control of forage for high producing animals is essential. Sperm production is
affected with DON level when is even below recommended standards for sows feed (cumulative
effect), and no clinical signs may appear. Commercial mycotoxin absorbers are not efficient for
such production.
PB  - Ecological Movement of the City of Novi Sad
C3  - Proceedings of XXIV International Eco-Conference on Safe Food
T1  - Effect of mycotoxins on porcine semen quality in artificial insemination centers
SP  - 271
EP  - 278
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_710
ER  - 

@conference{
author = "Milovanović, Aleksandar and Apić, Jelena and Barna, Tomislav and Živkov-Baloš, Milica and Prodanov Radulović, Jasna and Jakšić, Sandra and Maksimović, Nevena",
year = "2020",
abstract = "This paper describes escalation of sudden drop in semen quality in two boars' farm centers
affected by high mycotoxin level in forage (primarily by deoxynivalenol-DON). Barley and
wheat harvested in 2019 was generally indicated as most risky grains for animal health and
production and main source of DON. DON dominantly affected sperm total and progressive
motility (agglutination and astenospermia) as well as sperm chromatin structure, while acrosome
status and semen morphology were less sensitive to these changes. Bacterial control indicated
increase of bacterial presence. After forage replacement a period of 1-2 months is essential for
recovery of semen production, supported with prolonged vitamins and antibiotic treatment.
Routine control of forage for high producing animals is essential. Sperm production is
affected with DON level when is even below recommended standards for sows feed (cumulative
effect), and no clinical signs may appear. Commercial mycotoxin absorbers are not efficient for
such production.",
publisher = "Ecological Movement of the City of Novi Sad",
journal = "Proceedings of XXIV International Eco-Conference on Safe Food",
title = "Effect of mycotoxins on porcine semen quality in artificial insemination centers",
pages = "271-278",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_710"
}

Milovanović, A., Apić, J., Barna, T., Živkov-Baloš, M., Prodanov Radulović, J., Jakšić, S.,& Maksimović, N.. (2020). Effect of mycotoxins on porcine semen quality in artificial insemination centers. in Proceedings of XXIV International Eco-Conference on Safe Food
Ecological Movement of the City of Novi Sad., 271-278.
https://hdl.handle.net/21.15107/rcub_ristocar_710

Milovanović A, Apić J, Barna T, Živkov-Baloš M, Prodanov Radulović J, Jakšić S, Maksimović N. Effect of mycotoxins on porcine semen quality in artificial insemination centers. in Proceedings of XXIV International Eco-Conference on Safe Food. 2020;:271-278.
https://hdl.handle.net/21.15107/rcub_ristocar_710 .

Milovanović, Aleksandar, Apić, Jelena, Barna, Tomislav, Živkov-Baloš, Milica, Prodanov Radulović, Jasna, Jakšić, Sandra, Maksimović, Nevena, "Effect of mycotoxins on porcine semen quality in artificial insemination centers" in Proceedings of XXIV International Eco-Conference on Safe Food (2020):271-278,
https://hdl.handle.net/21.15107/rcub_ristocar_710 .

TY  - JOUR
AU  - Stojanov, Igor
AU  - Milovanović, Aleksandar
AU  - Barna, Tomislav
AU  - Prodanov Radulović, Jasna
AU  - Apić, Jelena
AU  - Stojanović, Dragica
AU  - Maksimović, Nevena
PY  - 2020
UR  - http://r.istocar.bg.ac.rs/handle/123456789/708
AB  - The aim of the study was to determine whether the bacteria from the environment and
from the mucous membrane of the boar prepuce have antimicrobial resistance and
whether the result obtained is similar/same to the bacteria that can be found in native
boar semen. The study addresses the problem of the presence of primarily resistant
bacterial strains in the boar sperm, which, due to their reduced sensitivity, cannot be
suppressed by antibiotics used in the semen dilution agent, as well as to emphasize the
importance of microbiological monitoring of the boar mucous membranes and ambient
surfaces before and during their exploitation. Such an examination could contribute to
the interchangeable design of the dilution agent for the boar semen relative to the
antibiotic content.Resistant strains of bacteria from prepuce swabs and swabs taken
from the facility, as well as from native boar semen were isolated. The presence of these
bacteria affected the quality of the semen. In conclusion, it should be pointed out that
bacterial monitoring of the prepuce and surface of the facility can indicate possible
problems related to the quality of semen, and that the design of the dilution agent for
boar semen should be adjusted to the established resistance of isolated bacteria.
PB  - Faculty of Veterinary Medicine, University of Belgrade, Serbia
T2  - Acta Veterinaria-Beograd
T1  - Antimicrobial resistance as a problem for the quality of boar semen
VL  - 7
IS  - 1
SP  - 136
EP  - 146
DO  - 10.2478/acve-2020-0010
ER  - 

@article{
author = "Stojanov, Igor and Milovanović, Aleksandar and Barna, Tomislav and Prodanov Radulović, Jasna and Apić, Jelena and Stojanović, Dragica and Maksimović, Nevena",
year = "2020",
abstract = "The aim of the study was to determine whether the bacteria from the environment and
from the mucous membrane of the boar prepuce have antimicrobial resistance and
whether the result obtained is similar/same to the bacteria that can be found in native
boar semen. The study addresses the problem of the presence of primarily resistant
bacterial strains in the boar sperm, which, due to their reduced sensitivity, cannot be
suppressed by antibiotics used in the semen dilution agent, as well as to emphasize the
importance of microbiological monitoring of the boar mucous membranes and ambient
surfaces before and during their exploitation. Such an examination could contribute to
the interchangeable design of the dilution agent for the boar semen relative to the
antibiotic content.Resistant strains of bacteria from prepuce swabs and swabs taken
from the facility, as well as from native boar semen were isolated. The presence of these
bacteria affected the quality of the semen. In conclusion, it should be pointed out that
bacterial monitoring of the prepuce and surface of the facility can indicate possible
problems related to the quality of semen, and that the design of the dilution agent for
boar semen should be adjusted to the established resistance of isolated bacteria.",
publisher = "Faculty of Veterinary Medicine, University of Belgrade, Serbia",
journal = "Acta Veterinaria-Beograd",
title = "Antimicrobial resistance as a problem for the quality of boar semen",
volume = "7",
number = "1",
pages = "136-146",
doi = "10.2478/acve-2020-0010"
}

Stojanov, I., Milovanović, A., Barna, T., Prodanov Radulović, J., Apić, J., Stojanović, D.,& Maksimović, N.. (2020). Antimicrobial resistance as a problem for the quality of boar semen. in Acta Veterinaria-Beograd
Faculty of Veterinary Medicine, University of Belgrade, Serbia., 7(1), 136-146.
https://doi.org/10.2478/acve-2020-0010

Stojanov I, Milovanović A, Barna T, Prodanov Radulović J, Apić J, Stojanović D, Maksimović N. Antimicrobial resistance as a problem for the quality of boar semen. in Acta Veterinaria-Beograd. 2020;7(1):136-146.
doi:10.2478/acve-2020-0010 .

Stojanov, Igor, Milovanović, Aleksandar, Barna, Tomislav, Prodanov Radulović, Jasna, Apić, Jelena, Stojanović, Dragica, Maksimović, Nevena, "Antimicrobial resistance as a problem for the quality of boar semen" in Acta Veterinaria-Beograd, 7, no. 1 (2020):136-146,
https://doi.org/10.2478/acve-2020-0010 . .

TY  - JOUR
AU  - Stojanov, Igor
AU  - Milovanović, Aleksandar
AU  - Ružić-Muslić, Dragana
AU  - Ratajac, Radomir
AU  - Živkov-Baloš, Milica
AU  - Maksimović, Nevena
AU  - Apić, Jelena
PY  - 2018
UR  - http://r.istocar.bg.ac.rs/handle/123456789/556
AB  - The treatment of reproductive disorders is based on the use of the available antibiotics or antiseptic agents. Prolonged or inadequate antibiotic treatment contributes to the development of antimicrobial resistances, while the use of antiseptics, particularly in high concentrations, leads to endometrial lesions and prolonging of the service period. The present study included monitoring of: 1) reproductive disorders of cows; 2) cytological, morphological, and bacteriological laboratory analyses; 3) susceptibility testing of isolated bacterial and reference strains to the EDTA-Tris (ethylene diamine tetraacetic acid-hydroxymethyl aminomethane) and chlorhexidine (CHX); and 4) an estimate of the time before onset of the new sexual cycle. The aim of the research was to determine whether the combination of EDTA-Tris and CHX in concentrations that are not harmful to the endometrium may result in the satisfactory treatment of sick animals and provide optimal service periods of reproductively active animals. Research results of in vitro and in vivo analysis showed that by using the combination of EDTA-Tris and CHX bactericidal effects were achieved at lower concentrations in comparison with the concentrations that had the same effect in the absence of EDTA-Tris. The application of CHX in a concentration of 0.05% in the treatment of endometritis 10 min after infusion of EDTA-Tris solution had a positive effect on both the involution of the uterus and acutization of chronic inflammatory processes, and it was also economically advantageous when compared to the cost of treatment and the possible presence of residues in the products.
PB  - Scientific Technical Research Council Turkey-Tubitak, Ankara
T2  - Turkish Journal of Veterinary & Animal Sciences
T1  - The application of EDTA-Tris and chlorhexidine in the treatment of endometritis as a replacement for antibiotic therapy in cows
VL  - 42
IS  - 1
SP  - 91
EP  - 96
DO  - 10.3906/vet-1703-74
ER  - 

@article{
author = "Stojanov, Igor and Milovanović, Aleksandar and Ružić-Muslić, Dragana and Ratajac, Radomir and Živkov-Baloš, Milica and Maksimović, Nevena and Apić, Jelena",
year = "2018",
abstract = "The treatment of reproductive disorders is based on the use of the available antibiotics or antiseptic agents. Prolonged or inadequate antibiotic treatment contributes to the development of antimicrobial resistances, while the use of antiseptics, particularly in high concentrations, leads to endometrial lesions and prolonging of the service period. The present study included monitoring of: 1) reproductive disorders of cows; 2) cytological, morphological, and bacteriological laboratory analyses; 3) susceptibility testing of isolated bacterial and reference strains to the EDTA-Tris (ethylene diamine tetraacetic acid-hydroxymethyl aminomethane) and chlorhexidine (CHX); and 4) an estimate of the time before onset of the new sexual cycle. The aim of the research was to determine whether the combination of EDTA-Tris and CHX in concentrations that are not harmful to the endometrium may result in the satisfactory treatment of sick animals and provide optimal service periods of reproductively active animals. Research results of in vitro and in vivo analysis showed that by using the combination of EDTA-Tris and CHX bactericidal effects were achieved at lower concentrations in comparison with the concentrations that had the same effect in the absence of EDTA-Tris. The application of CHX in a concentration of 0.05% in the treatment of endometritis 10 min after infusion of EDTA-Tris solution had a positive effect on both the involution of the uterus and acutization of chronic inflammatory processes, and it was also economically advantageous when compared to the cost of treatment and the possible presence of residues in the products.",
publisher = "Scientific Technical Research Council Turkey-Tubitak, Ankara",
journal = "Turkish Journal of Veterinary & Animal Sciences",
title = "The application of EDTA-Tris and chlorhexidine in the treatment of endometritis as a replacement for antibiotic therapy in cows",
volume = "42",
number = "1",
pages = "91-96",
doi = "10.3906/vet-1703-74"
}

Stojanov, I., Milovanović, A., Ružić-Muslić, D., Ratajac, R., Živkov-Baloš, M., Maksimović, N.,& Apić, J.. (2018). The application of EDTA-Tris and chlorhexidine in the treatment of endometritis as a replacement for antibiotic therapy in cows. in Turkish Journal of Veterinary & Animal Sciences
Scientific Technical Research Council Turkey-Tubitak, Ankara., 42(1), 91-96.
https://doi.org/10.3906/vet-1703-74

Stojanov I, Milovanović A, Ružić-Muslić D, Ratajac R, Živkov-Baloš M, Maksimović N, Apić J. The application of EDTA-Tris and chlorhexidine in the treatment of endometritis as a replacement for antibiotic therapy in cows. in Turkish Journal of Veterinary & Animal Sciences. 2018;42(1):91-96.
doi:10.3906/vet-1703-74 .

Stojanov, Igor, Milovanović, Aleksandar, Ružić-Muslić, Dragana, Ratajac, Radomir, Živkov-Baloš, Milica, Maksimović, Nevena, Apić, Jelena, "The application of EDTA-Tris and chlorhexidine in the treatment of endometritis as a replacement for antibiotic therapy in cows" in Turkish Journal of Veterinary & Animal Sciences, 42, no. 1 (2018):91-96,
https://doi.org/10.3906/vet-1703-74 . .

TY  - GEN
AU  - Stojanov, Igor
AU  - Milovanović, Aleksandar
AU  - Ružić-Muslić, Dragana
AU  - Ratajac, Radomir
AU  - Živkov Baloš, Milica
AU  - Maksimović, Nevena
AU  - Apić, Jelena
PY  - 2017
UR  - http://r.istocar.bg.ac.rs/handle/123456789/851
AB  - Savremeni pristup u stočarskoj proizvodnji mora da obuhvati kreiranje tehnoloških postupaka koji omogućavaju dobijanje proizvoda koji zadovaljavaju principe ekonomičnosti, kao i potrebu obzebeđivanja biološki vredne i bezbedne hrane. Poseban zadatak nauke je prepoznavanje nedostataka u postojećim rešenjima i dizajniranje novih postupaka koji će uspostavljanjem novih modela omogućiti ostvarenje postavljenih ciljeva. Sa obzirom, da tehnološki postupci, kroz vreme, divergiraju jer se rasčlanjivanjem pojedinih faza proizvodnje lakše uočavaju potencijalni problemi, može se reči da se iz istih razloga javlja i njihovo usložnjavanje, što uglavnom zavisi od stepena razvoja zemlje. Međutim, neke pojave, kao razvoj i širenje antimikrobne rezistencije, prisutne su u svim zemljama gde stočarstvo ima važnu ulogu u prehrambenoj industriji. Pored spomenute činjenice, potreba rešavanja nekih zdravstvenih poremećaja, kao što su reproduktivni problemi, zahtevaju iznalaženje novih postupaka lečenja.
U našoj zemlji postupak lečenja pojedinih formi reproduktivnih poremećaja zasniva se na upotrebi dostupnih antibiotika ili antiseptičnih sredstava. Često se pristupa upotrebi ovih preparata bez valjane kliničke i laboratorijske analize. Ovakav pristup terapiji sa jedne strane doprinosi pojavi antimikrobne rezistencije zbog korišćenja antibiotika i onda kada to nije opravdano, dok sa druge strane upotreba antiseptika, posebno u visokim koncetracijam, dovodi do ozlede endometrijuma i prolongiranja servis perioda. Pojava rezistencije dovodi do neželjenih konsekvenci kako u veterinarskoj tako i humanoj medici jer se gubi glavno oružije u borbi protiv mikroorganizama i sve češće ostaje bez opcije pri izboru antimikrobnog leka. Prolongiranje servis perioda kod krava dovodi do materijalnih gubitaka jer se gubi mogućnost dobijanja jednog teleta godišenje, a u težim slučajevima, često se gubi i sama krava.
Rezultati ovog istraživanja u in vitro uslovima, su pokazali da se primenom kombnacije EDTA-Tris i hlorheksidin glukunata, postiže baktericidni efekat u nižim koncetracijama u poređenju sa koncetracijama koje su imale isti efekat bez prisustva EDTA-Tris. Kod svih ispitujućih sojeva, posle izlaganja bakterijskih suspenzija delovanju EDTA-Tris, koji kao helat menja permeablnost ćelijskog zida, potvrđeno je znatno povećanje osetljivost na hlorheksidin što se manifestovalo smanjenjem potrebne koncetracije ovoga antiseptika koji je imao baktericidno dejstvo.
Primena hlorheksidin-glukonata u koncentraciji od 0,05% za tretman endometritisa 10 minuta nakon infuzije rastvora EDTA-Tris ima povoljno delovanje kako na involuciju materice, tako i na akutizaciju hroničnih upalnih procesa. Ovaj vid terapije u kliničkim ogledu (postizanje steonosti kao krajni cilj) ima komparativne prednosti u odnosu na postojeće tretmane u povoljnoj ceni preparata (oko 220 dinara - 1,78 EU centi po ispiranju), nema karencu za mleko i meso a oporavak tokom involucije nije značajnije narušen. Efekat kod krava sa hroničnim endometritisima je u rangu standardnog uspeha terapije postojećim terapeuticima, ili bolji, u zavisnosti od stepena upalnog procesa.
Implementacija dobijenih rezultata u ovom tehničkom rešenju doprinosi rešavanju važnog segmetna u stočarskoj proizvodnji vezanog za reprodukciju. Mogućnost korišćenja optimalnih reproduktivnih sposobnosti i obezbeđivanje dobijajna jednog teleta godišnje omogućava ekonomičnost proizvodnje. Pored toga upotreba antibiotika se smanjuje čime se direktno utiče na smanjenje pojave i širenja mikrobne rezistencije u stočarstvu a indirektno i u humanoj medicini.
T1  - Novi tehnološki postupak lečenja infekcija reproduktivnog trakta krava primenom edta-tris i hlorheksidina - Tehničko rešenje
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_851
ER  - 

@misc{
author = "Stojanov, Igor and Milovanović, Aleksandar and Ružić-Muslić, Dragana and Ratajac, Radomir and Živkov Baloš, Milica and Maksimović, Nevena and Apić, Jelena",
year = "2017",
abstract = "Savremeni pristup u stočarskoj proizvodnji mora da obuhvati kreiranje tehnoloških postupaka koji omogućavaju dobijanje proizvoda koji zadovaljavaju principe ekonomičnosti, kao i potrebu obzebeđivanja biološki vredne i bezbedne hrane. Poseban zadatak nauke je prepoznavanje nedostataka u postojećim rešenjima i dizajniranje novih postupaka koji će uspostavljanjem novih modela omogućiti ostvarenje postavljenih ciljeva. Sa obzirom, da tehnološki postupci, kroz vreme, divergiraju jer se rasčlanjivanjem pojedinih faza proizvodnje lakše uočavaju potencijalni problemi, može se reči da se iz istih razloga javlja i njihovo usložnjavanje, što uglavnom zavisi od stepena razvoja zemlje. Međutim, neke pojave, kao razvoj i širenje antimikrobne rezistencije, prisutne su u svim zemljama gde stočarstvo ima važnu ulogu u prehrambenoj industriji. Pored spomenute činjenice, potreba rešavanja nekih zdravstvenih poremećaja, kao što su reproduktivni problemi, zahtevaju iznalaženje novih postupaka lečenja.
U našoj zemlji postupak lečenja pojedinih formi reproduktivnih poremećaja zasniva se na upotrebi dostupnih antibiotika ili antiseptičnih sredstava. Često se pristupa upotrebi ovih preparata bez valjane kliničke i laboratorijske analize. Ovakav pristup terapiji sa jedne strane doprinosi pojavi antimikrobne rezistencije zbog korišćenja antibiotika i onda kada to nije opravdano, dok sa druge strane upotreba antiseptika, posebno u visokim koncetracijam, dovodi do ozlede endometrijuma i prolongiranja servis perioda. Pojava rezistencije dovodi do neželjenih konsekvenci kako u veterinarskoj tako i humanoj medici jer se gubi glavno oružije u borbi protiv mikroorganizama i sve češće ostaje bez opcije pri izboru antimikrobnog leka. Prolongiranje servis perioda kod krava dovodi do materijalnih gubitaka jer se gubi mogućnost dobijanja jednog teleta godišenje, a u težim slučajevima, često se gubi i sama krava.
Rezultati ovog istraživanja u in vitro uslovima, su pokazali da se primenom kombnacije EDTA-Tris i hlorheksidin glukunata, postiže baktericidni efekat u nižim koncetracijama u poređenju sa koncetracijama koje su imale isti efekat bez prisustva EDTA-Tris. Kod svih ispitujućih sojeva, posle izlaganja bakterijskih suspenzija delovanju EDTA-Tris, koji kao helat menja permeablnost ćelijskog zida, potvrđeno je znatno povećanje osetljivost na hlorheksidin što se manifestovalo smanjenjem potrebne koncetracije ovoga antiseptika koji je imao baktericidno dejstvo.
Primena hlorheksidin-glukonata u koncentraciji od 0,05% za tretman endometritisa 10 minuta nakon infuzije rastvora EDTA-Tris ima povoljno delovanje kako na involuciju materice, tako i na akutizaciju hroničnih upalnih procesa. Ovaj vid terapije u kliničkim ogledu (postizanje steonosti kao krajni cilj) ima komparativne prednosti u odnosu na postojeće tretmane u povoljnoj ceni preparata (oko 220 dinara - 1,78 EU centi po ispiranju), nema karencu za mleko i meso a oporavak tokom involucije nije značajnije narušen. Efekat kod krava sa hroničnim endometritisima je u rangu standardnog uspeha terapije postojećim terapeuticima, ili bolji, u zavisnosti od stepena upalnog procesa.
Implementacija dobijenih rezultata u ovom tehničkom rešenju doprinosi rešavanju važnog segmetna u stočarskoj proizvodnji vezanog za reprodukciju. Mogućnost korišćenja optimalnih reproduktivnih sposobnosti i obezbeđivanje dobijajna jednog teleta godišnje omogućava ekonomičnost proizvodnje. Pored toga upotreba antibiotika se smanjuje čime se direktno utiče na smanjenje pojave i širenja mikrobne rezistencije u stočarstvu a indirektno i u humanoj medicini.",
title = "Novi tehnološki postupak lečenja infekcija reproduktivnog trakta krava primenom edta-tris i hlorheksidina - Tehničko rešenje",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_851"
}

Stojanov, I., Milovanović, A., Ružić-Muslić, D., Ratajac, R., Živkov Baloš, M., Maksimović, N.,& Apić, J.. (2017). Novi tehnološki postupak lečenja infekcija reproduktivnog trakta krava primenom edta-tris i hlorheksidina - Tehničko rešenje. .
https://hdl.handle.net/21.15107/rcub_ristocar_851

Stojanov I, Milovanović A, Ružić-Muslić D, Ratajac R, Živkov Baloš M, Maksimović N, Apić J. Novi tehnološki postupak lečenja infekcija reproduktivnog trakta krava primenom edta-tris i hlorheksidina - Tehničko rešenje. 2017;.
https://hdl.handle.net/21.15107/rcub_ristocar_851 .

Stojanov, Igor, Milovanović, Aleksandar, Ružić-Muslić, Dragana, Ratajac, Radomir, Živkov Baloš, Milica, Maksimović, Nevena, Apić, Jelena, "Novi tehnološki postupak lečenja infekcija reproduktivnog trakta krava primenom edta-tris i hlorheksidina - Tehničko rešenje" (2017),
https://hdl.handle.net/21.15107/rcub_ristocar_851 .