TY  - CONF
AU  - Petrova, Viktoria
AU  - Petrov, Georgi
AU  - Cekić, Bogdan
AU  - Andreeva, Madlena
PY  - 2022-12
UR  - http://r.istocar.bg.ac.rs/handle/123456789/812
AB  - Sheep (Ovies aries) are mainly seasonal polycyclic animals. Sperm production is influenced by many factors, including daylight length, climate temperature, humidity, and melatonin secretion, with females becoming fertile in early autumn and remaining fertile through mid-winter (October-February). Seminal plasma is a complex fluid that is functional modulator of sperm function known to inhibit and stimulate sperm function and viability. Some seminal plasma proteins are capable of binding to the sperm plasma membrane and have an important role in sperm capacitation and egg fertilization. A variety of enzymes in the seminal plasma protect the sperm membrane from ROS-induced damage and lipid peroxidation through the activity of the antioxidant enzyme defense system. Regarding this, the aim of our study is to determine the biochemical changes that occur during the breeding campaign of the species Ovies aries and beyond. To fulfill the set goal, we have examined 6 ejaculates spectrophotometrically. As a result, we found higher antioxidant enzyme protection outside the insemination campaign in glutathione (GSH) concentration and glutathione peroxidase (GPx) activity and non-significant differences in glutathione reductase (GR) activity. The enzyme activities of lactate dehydrogenase (LDH) and alkaline phosphatase (ALP) were higher during breeding season of the animals. In conclusion, a higher protective antioxidant system may help ensure adequate sperm fertilization potential when reproductive conditions are suboptimal. However, further research is needed to determine the mechanisms of action of the antioxidant enzyme and the relationships between indicators for determining sperm quality.
C3  - Book of abstracts of National Scientific conference "75 years Institute of Neurobilogy" 15-16.december, Sofia, Bulgaria
T1  - Seasonal variations in enzyme activity in Ovies aries seminal plasma
SP  - 46
EP  - 46
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_812
ER  - 

@conference{
author = "Petrova, Viktoria and Petrov, Georgi and Cekić, Bogdan and Andreeva, Madlena",
year = "2022-12",
abstract = "Sheep (Ovies aries) are mainly seasonal polycyclic animals. Sperm production is influenced by many factors, including daylight length, climate temperature, humidity, and melatonin secretion, with females becoming fertile in early autumn and remaining fertile through mid-winter (October-February). Seminal plasma is a complex fluid that is functional modulator of sperm function known to inhibit and stimulate sperm function and viability. Some seminal plasma proteins are capable of binding to the sperm plasma membrane and have an important role in sperm capacitation and egg fertilization. A variety of enzymes in the seminal plasma protect the sperm membrane from ROS-induced damage and lipid peroxidation through the activity of the antioxidant enzyme defense system. Regarding this, the aim of our study is to determine the biochemical changes that occur during the breeding campaign of the species Ovies aries and beyond. To fulfill the set goal, we have examined 6 ejaculates spectrophotometrically. As a result, we found higher antioxidant enzyme protection outside the insemination campaign in glutathione (GSH) concentration and glutathione peroxidase (GPx) activity and non-significant differences in glutathione reductase (GR) activity. The enzyme activities of lactate dehydrogenase (LDH) and alkaline phosphatase (ALP) were higher during breeding season of the animals. In conclusion, a higher protective antioxidant system may help ensure adequate sperm fertilization potential when reproductive conditions are suboptimal. However, further research is needed to determine the mechanisms of action of the antioxidant enzyme and the relationships between indicators for determining sperm quality.",
journal = "Book of abstracts of National Scientific conference "75 years Institute of Neurobilogy" 15-16.december, Sofia, Bulgaria",
title = "Seasonal variations in enzyme activity in Ovies aries seminal plasma",
pages = "46-46",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_812"
}

Petrova, V., Petrov, G., Cekić, B.,& Andreeva, M.. (2022-12). Seasonal variations in enzyme activity in Ovies aries seminal plasma. in Book of abstracts of National Scientific conference "75 years Institute of Neurobilogy" 15-16.december, Sofia, Bulgaria, 46-46.
https://hdl.handle.net/21.15107/rcub_ristocar_812

Petrova V, Petrov G, Cekić B, Andreeva M. Seasonal variations in enzyme activity in Ovies aries seminal plasma. in Book of abstracts of National Scientific conference "75 years Institute of Neurobilogy" 15-16.december, Sofia, Bulgaria. 2022;:46-46.
https://hdl.handle.net/21.15107/rcub_ristocar_812 .

Petrova, Viktoria, Petrov, Georgi, Cekić, Bogdan, Andreeva, Madlena, "Seasonal variations in enzyme activity in Ovies aries seminal plasma" in Book of abstracts of National Scientific conference "75 years Institute of Neurobilogy" 15-16.december, Sofia, Bulgaria (2022-12):46-46,
https://hdl.handle.net/21.15107/rcub_ristocar_812 .

TY  - JOUR
AU  - Cekić, Bogdan
AU  - Kanzova, Hristiyana
AU  - Petrov, Georgi
AU  - Maksimović, Nevena
AU  - Ćosić, Ivan
AU  - Milovanović, Aleksandar
PY  - 2022
UR  - http://r.istocar.bg.ac.rs/handle/123456789/797
AB  - The current study aimed to examine the percentage changes of
viability sperm and the activity of the enzymes LDH, ALP, GOT / AST, GPT /
ALT in the sperm plasma of Lacaune rams, before and after cryopreservation. For
this purpose, five rams were examined, and two ejaculates were obtained from each
ram. Ejaculates are collected by the method of artificial vagina, during the
insemination campaign. All ejaculates were diluted with a 6AG extender and
frozen by the Cassou’s sequin method. Sperm viability was determined by eosin
and nigrosine smears, and enzyme activity was examined spectrophotometrically.
As a result, the percentage of vital sperm after cryopreservation decreased by 15%
(P ≤ 0.001). The freezing and thawing process also reduced the activity of the
enzymes LDH, ALP, GOT / AST and GPT / ALP. In conclusion, the observed
enzymes, in relation to sperm vitality, could be used as indicators to optimize the
protocols for cryopreservation of ram’s sperm.
AB  - Istraživanje je imalo za cilj ispitivanje promena u procentu vijabilnih
spermatozoida i promena u aktivnosti enzima LDH, ALP, GOT / AST, GPT / ALT
u seminalnoj plazmi ovnova rase Lakon, pre i nakon krioprezervacije. Za tu svrhu,
ispitivana je sperma pet ovnova, sa dva ejakulata po ovnu. Ejakulati su dobijeni
metodom veštačke vagine, u toku sezone parenja. Svi ejakulati su razređeni
razređivačem 6AG i zamrznuti po metodi Cassou sequin. Vijabilnost
spermatozoida je određena na osnovu razmaza sa eozinom i nigrozinom, a
aktivnost enzima je određena spektrofotometrijski. Rezultati istraživanja pokazuju
da je udeo vitalnih spermatozoida nakon krioprezervacije smanjen za 15% (P ≤
0.001). Procesi zamrzavanja i odmrzavanja takođe smanjuju i aktivnost enzima
LDH, ALP, GOT/ AST i GPT / ALP. Ispitivani enzimi, združeno sa vitalnošću
spermatozoida, mogu da se koriste kao markeri za optimizaciju stope hlađenja i
koraka u procesima zamrzavanja i odmrzavanja sperme ovnova.
PB  - Institute for Animal Husbandry, Belgrade-Zemun, Serbia
T2  - Biotechnology in animal husbandry
T1  - Analysis of vitality and biochemical parameters in freeze-thawed seminal plasma of rams
VL  - 38
IS  - 1
SP  - 31
EP  - 39
DO  - 10.2298/BAH2201031C
ER  - 

@article{
author = "Cekić, Bogdan and Kanzova, Hristiyana and Petrov, Georgi and Maksimović, Nevena and Ćosić, Ivan and Milovanović, Aleksandar",
year = "2022",
abstract = "The current study aimed to examine the percentage changes of
viability sperm and the activity of the enzymes LDH, ALP, GOT / AST, GPT /
ALT in the sperm plasma of Lacaune rams, before and after cryopreservation. For
this purpose, five rams were examined, and two ejaculates were obtained from each
ram. Ejaculates are collected by the method of artificial vagina, during the
insemination campaign. All ejaculates were diluted with a 6AG extender and
frozen by the Cassou’s sequin method. Sperm viability was determined by eosin
and nigrosine smears, and enzyme activity was examined spectrophotometrically.
As a result, the percentage of vital sperm after cryopreservation decreased by 15%
(P ≤ 0.001). The freezing and thawing process also reduced the activity of the
enzymes LDH, ALP, GOT / AST and GPT / ALP. In conclusion, the observed
enzymes, in relation to sperm vitality, could be used as indicators to optimize the
protocols for cryopreservation of ram’s sperm., Istraživanje je imalo za cilj ispitivanje promena u procentu vijabilnih
spermatozoida i promena u aktivnosti enzima LDH, ALP, GOT / AST, GPT / ALT
u seminalnoj plazmi ovnova rase Lakon, pre i nakon krioprezervacije. Za tu svrhu,
ispitivana je sperma pet ovnova, sa dva ejakulata po ovnu. Ejakulati su dobijeni
metodom veštačke vagine, u toku sezone parenja. Svi ejakulati su razređeni
razređivačem 6AG i zamrznuti po metodi Cassou sequin. Vijabilnost
spermatozoida je određena na osnovu razmaza sa eozinom i nigrozinom, a
aktivnost enzima je određena spektrofotometrijski. Rezultati istraživanja pokazuju
da je udeo vitalnih spermatozoida nakon krioprezervacije smanjen za 15% (P ≤
0.001). Procesi zamrzavanja i odmrzavanja takođe smanjuju i aktivnost enzima
LDH, ALP, GOT/ AST i GPT / ALP. Ispitivani enzimi, združeno sa vitalnošću
spermatozoida, mogu da se koriste kao markeri za optimizaciju stope hlađenja i
koraka u procesima zamrzavanja i odmrzavanja sperme ovnova.",
publisher = "Institute for Animal Husbandry, Belgrade-Zemun, Serbia",
journal = "Biotechnology in animal husbandry",
title = "Analysis of vitality and biochemical parameters in freeze-thawed seminal plasma of rams",
volume = "38",
number = "1",
pages = "31-39",
doi = "10.2298/BAH2201031C"
}

Cekić, B., Kanzova, H., Petrov, G., Maksimović, N., Ćosić, I.,& Milovanović, A.. (2022). Analysis of vitality and biochemical parameters in freeze-thawed seminal plasma of rams. in Biotechnology in animal husbandry
Institute for Animal Husbandry, Belgrade-Zemun, Serbia., 38(1), 31-39.
https://doi.org/10.2298/BAH2201031C

Cekić B, Kanzova H, Petrov G, Maksimović N, Ćosić I, Milovanović A. Analysis of vitality and biochemical parameters in freeze-thawed seminal plasma of rams. in Biotechnology in animal husbandry. 2022;38(1):31-39.
doi:10.2298/BAH2201031C .

Cekić, Bogdan, Kanzova, Hristiyana, Petrov, Georgi, Maksimović, Nevena, Ćosić, Ivan, Milovanović, Aleksandar, "Analysis of vitality and biochemical parameters in freeze-thawed seminal plasma of rams" in Biotechnology in animal husbandry, 38, no. 1 (2022):31-39,
https://doi.org/10.2298/BAH2201031C . .