TY  - CONF
AU  - Stanojković, Aleksandar
AU  - Stanišić, Nikola
AU  - Delić, Nikola
AU  - Bošnjak, Ivan
AU  - Mandić, Violeta
AU  - Stanojković-Sebić, Aleksandra
AU  - Nišavić, Jakov
PY  - 2023-10-04
UR  - http://r.istocar.bg.ac.rs/handle/123456789/896
AB  - Streptococcus suis infection is one of the major health problems in the
swine industry worldwide. During the last decade, the number of reported human
cases due to S. suis has dramatically increased, and while most sporadic human
cases of infection appear to be due to close occupational contact with pigs/pork
products. S. suis infection is considered to be multifactorial, with transition from
subclinical to clinical that depends on many factors. These factors can be divided in
two groups, host-based and external factors. Pathogenesis of S. suis infection can
be divided into 4 phases: adherence to and colonisation of mucosal and epithelial
surfaces, invasion into deeper tissues and entering the bloodstream, crossing bloodbrain
barrier and inflammation. S. suis virulence-associated factors are divided into
the following 4 groups: surface/secreted elements, enzymes (such as including
proteases), transcription factors and regulatory systems and other factors (such as
transporting and secreting systems). Therefore significant research support is needed
to obtain a vaccine as a valuable and universal protection against disease caused by S.
suis strains and thus national and international support will be crucial for the aim many
researchers hope for.
PB  - Institute for Animal Husbandry, Belgrade - Zemun
T1  - STREPTOCOCCUS SUIS, TWO-FACED GAME CHANGER
SP  - 251
EP  - 266
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_896
ER  - 

@conference{
author = "Stanojković, Aleksandar and Stanišić, Nikola and Delić, Nikola and Bošnjak, Ivan and Mandić, Violeta and Stanojković-Sebić, Aleksandra and Nišavić, Jakov",
year = "2023-10-04",
abstract = "Streptococcus suis infection is one of the major health problems in the
swine industry worldwide. During the last decade, the number of reported human
cases due to S. suis has dramatically increased, and while most sporadic human
cases of infection appear to be due to close occupational contact with pigs/pork
products. S. suis infection is considered to be multifactorial, with transition from
subclinical to clinical that depends on many factors. These factors can be divided in
two groups, host-based and external factors. Pathogenesis of S. suis infection can
be divided into 4 phases: adherence to and colonisation of mucosal and epithelial
surfaces, invasion into deeper tissues and entering the bloodstream, crossing bloodbrain
barrier and inflammation. S. suis virulence-associated factors are divided into
the following 4 groups: surface/secreted elements, enzymes (such as including
proteases), transcription factors and regulatory systems and other factors (such as
transporting and secreting systems). Therefore significant research support is needed
to obtain a vaccine as a valuable and universal protection against disease caused by S.
suis strains and thus national and international support will be crucial for the aim many
researchers hope for.",
publisher = "Institute for Animal Husbandry, Belgrade - Zemun",
title = "STREPTOCOCCUS SUIS, TWO-FACED GAME CHANGER",
pages = "251-266",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_896"
}

Stanojković, A., Stanišić, N., Delić, N., Bošnjak, I., Mandić, V., Stanojković-Sebić, A.,& Nišavić, J.. (2023-10-04). STREPTOCOCCUS SUIS, TWO-FACED GAME CHANGER. 
Institute for Animal Husbandry, Belgrade - Zemun., 251-266.
https://hdl.handle.net/21.15107/rcub_ristocar_896

Stanojković A, Stanišić N, Delić N, Bošnjak I, Mandić V, Stanojković-Sebić A, Nišavić J. STREPTOCOCCUS SUIS, TWO-FACED GAME CHANGER. 2023;:251-266.
https://hdl.handle.net/21.15107/rcub_ristocar_896 .

Stanojković, Aleksandar, Stanišić, Nikola, Delić, Nikola, Bošnjak, Ivan, Mandić, Violeta, Stanojković-Sebić, Aleksandra, Nišavić, Jakov, "STREPTOCOCCUS SUIS, TWO-FACED GAME CHANGER" (2023-10-04):251-266,
https://hdl.handle.net/21.15107/rcub_ristocar_896 .

TY  - JOUR
AU  - Nišavić, Jakov
AU  - Radalj, Andrea
AU  - Milić, Nenad
AU  - Živulj, Aleksandar
AU  - Benković, Damir
AU  - Stanojković, Aleksandar
AU  - Prošić, Isidora
PY  - 2021-12-30
UR  - http://r.istocar.bg.ac.rs/handle/123456789/969
AB  - Wild boars are one of the widest-ranging mammals worldwide and represent reservoirs for many important viruses. Disease outbreaks in domestic swine are often described as a consequence of contact with wild boars, and traditional rearing conditions are a particular risk factor. Examples of such diseases include classical swine fever (CSF), African swine fever (ASF), Aujeszky’s disease (AD), and diseases caused by porcine circoviruses and parvoviruses. Some viral infections causing high mortality rates are easily noticeable and thus reported, though many viruses infecting wildlife are insidious impacting survival rates and reproduction in wild animals. Samples from wild boars for laboratory testing are usually collected postmortem and include various tissues or blood sera. The recovery of viable viruses during virus isolation depends on the virus species and the condition of the sample. Since this method does not yield timely results, most diagnostic procedures are based on PCR or antigen detection methods. Serological surveys are inexpensive and appropriate for prevalence studies. When interpreting the results of diagnostic tests, both virus and host characteristics, and the epizootiological situation must be accounted for. Disease control techniques such as fencing or feeding wild boars cause animal aggregation and give rise to population density which favors pathogen maintenance in the environment. Hunting reduces the number of susceptible animals and is helpful as an additional control measure and for sampling. Available data on infectious disease dynamics in wild boars is scarce, and constant knowledge improvement on pathogenesis, clinical symptoms, risk factors, and adequate control measures are required.
PB  - Institut za stočarstvo, Beograd-Zemun
T2  - Biotechnology in Animal Husbandry
T1  - A REVIEW OF SOME IMPORTANT VIRAL DISEASES OF WILD BOARS
VL  - 37
IS  - 4
SP  - 235
EP  - 254
DO  - 10.2298/BAH2104235N
ER  - 

@article{
author = "Nišavić, Jakov and Radalj, Andrea and Milić, Nenad and Živulj, Aleksandar and Benković, Damir and Stanojković, Aleksandar and Prošić, Isidora",
year = "2021-12-30",
abstract = "Wild boars are one of the widest-ranging mammals worldwide and represent reservoirs for many important viruses. Disease outbreaks in domestic swine are often described as a consequence of contact with wild boars, and traditional rearing conditions are a particular risk factor. Examples of such diseases include classical swine fever (CSF), African swine fever (ASF), Aujeszky’s disease (AD), and diseases caused by porcine circoviruses and parvoviruses. Some viral infections causing high mortality rates are easily noticeable and thus reported, though many viruses infecting wildlife are insidious impacting survival rates and reproduction in wild animals. Samples from wild boars for laboratory testing are usually collected postmortem and include various tissues or blood sera. The recovery of viable viruses during virus isolation depends on the virus species and the condition of the sample. Since this method does not yield timely results, most diagnostic procedures are based on PCR or antigen detection methods. Serological surveys are inexpensive and appropriate for prevalence studies. When interpreting the results of diagnostic tests, both virus and host characteristics, and the epizootiological situation must be accounted for. Disease control techniques such as fencing or feeding wild boars cause animal aggregation and give rise to population density which favors pathogen maintenance in the environment. Hunting reduces the number of susceptible animals and is helpful as an additional control measure and for sampling. Available data on infectious disease dynamics in wild boars is scarce, and constant knowledge improvement on pathogenesis, clinical symptoms, risk factors, and adequate control measures are required.",
publisher = "Institut za stočarstvo, Beograd-Zemun",
journal = "Biotechnology in Animal Husbandry",
title = "A REVIEW OF SOME IMPORTANT VIRAL DISEASES OF WILD BOARS",
volume = "37",
number = "4",
pages = "235-254",
doi = "10.2298/BAH2104235N"
}

Nišavić, J., Radalj, A., Milić, N., Živulj, A., Benković, D., Stanojković, A.,& Prošić, I.. (2021-12-30). A REVIEW OF SOME IMPORTANT VIRAL DISEASES OF WILD BOARS. in Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd-Zemun., 37(4), 235-254.
https://doi.org/10.2298/BAH2104235N

Nišavić J, Radalj A, Milić N, Živulj A, Benković D, Stanojković A, Prošić I. A REVIEW OF SOME IMPORTANT VIRAL DISEASES OF WILD BOARS. in Biotechnology in Animal Husbandry. 2021;37(4):235-254.
doi:10.2298/BAH2104235N .

Nišavić, Jakov, Radalj, Andrea, Milić, Nenad, Živulj, Aleksandar, Benković, Damir, Stanojković, Aleksandar, Prošić, Isidora, "A REVIEW OF SOME IMPORTANT VIRAL DISEASES OF WILD BOARS" in Biotechnology in Animal Husbandry, 37, no. 4 (2021-12-30):235-254,
https://doi.org/10.2298/BAH2104235N . .

TY  - CONF
AU  - Stanojković, Aleksandar
AU  - Radović, Čedomir
AU  - Stanojković-Sebić, Aleksandra
AU  - Gogić, Marija
AU  - Mandić, Violeta
AU  - Nišavić, Jakov
AU  - Petričević, Maja
PY  - 2019
UR  - http://r.istocar.bg.ac.rs/handle/123456789/676
AB  - The goal of this study was to determine antimicrobial
susceptibility of 34 Streptococcus suis strains isolated from healthy and diseased
pigs from two pig farms. Disk diffusion was the method used to asses antibacterial
susceptibility of S. suis according to CLSI (Clinical and Laboratory Standards
Institute) and EUCAST standard protocols. Overnight cultures of S. suis grown on
Colombia 5% blood agar (HIMEDIA, India) were suspended in Todd Hewitt broth
(Oxoid Limited, England) and suspension was adjusted to 0,5 McFarland standard.
Using sterile swabs suspension was spreaded to Mueller Hinton agar supplemented
with 5% defibrinated sheep blood, after which standadized discs containing
antimicrobial substances were placed on the agar surface. Plates were incubated at
37 °C in aerobic conditions usually for 18 hours, but no more than 24 hours after
which diameter of S. suis inhibition zones were measured. Among 34 isolated S.
suis strains all were resistant to tetracycline (TET), lincomycin (L) and
clindamycin (CC). Susceptibility was very low to sulfamethoxazole-trimethoprim
(SXT) combination (94,1% resistance), 94,1% of strains were sensitive to
penicillin G (P), amoxicillin (AMX), erythromycin (E), enrofloxacin (ENR) and
azithromycin (AZM) while sensitivity to cephalosporins of the first (cephalexin-
CN), third (cefotaxime – CTX, ceftriaxone - CRO) and fourth ( cefepime-FEP)
generation, as well as the sensitivity to florfenicol (FFC) and vancomycin (VAN)
was absolute (100%). Therefore we can conclude that cephalosporins are very
reasonable choice in treatment of human cases of S. suis meningitis but also as a
treatment of choice for the S .suis pig septicemia.
PB  - Institute for Animal Husbandry, Belgrade-Zemun
C3  - Proceedings of the 12th International Symposium Modern Trends in Livestock Production, Belgrade
T1  - Antimicrobial susceptibility testing of streptococcus suis isolates to common antibiotics used in pig farms
SP  - 148
EP  - 154
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_676
ER  - 

@conference{
author = "Stanojković, Aleksandar and Radović, Čedomir and Stanojković-Sebić, Aleksandra and Gogić, Marija and Mandić, Violeta and Nišavić, Jakov and Petričević, Maja",
year = "2019",
abstract = "The goal of this study was to determine antimicrobial
susceptibility of 34 Streptococcus suis strains isolated from healthy and diseased
pigs from two pig farms. Disk diffusion was the method used to asses antibacterial
susceptibility of S. suis according to CLSI (Clinical and Laboratory Standards
Institute) and EUCAST standard protocols. Overnight cultures of S. suis grown on
Colombia 5% blood agar (HIMEDIA, India) were suspended in Todd Hewitt broth
(Oxoid Limited, England) and suspension was adjusted to 0,5 McFarland standard.
Using sterile swabs suspension was spreaded to Mueller Hinton agar supplemented
with 5% defibrinated sheep blood, after which standadized discs containing
antimicrobial substances were placed on the agar surface. Plates were incubated at
37 °C in aerobic conditions usually for 18 hours, but no more than 24 hours after
which diameter of S. suis inhibition zones were measured. Among 34 isolated S.
suis strains all were resistant to tetracycline (TET), lincomycin (L) and
clindamycin (CC). Susceptibility was very low to sulfamethoxazole-trimethoprim
(SXT) combination (94,1% resistance), 94,1% of strains were sensitive to
penicillin G (P), amoxicillin (AMX), erythromycin (E), enrofloxacin (ENR) and
azithromycin (AZM) while sensitivity to cephalosporins of the first (cephalexin-
CN), third (cefotaxime – CTX, ceftriaxone - CRO) and fourth ( cefepime-FEP)
generation, as well as the sensitivity to florfenicol (FFC) and vancomycin (VAN)
was absolute (100%). Therefore we can conclude that cephalosporins are very
reasonable choice in treatment of human cases of S. suis meningitis but also as a
treatment of choice for the S .suis pig septicemia.",
publisher = "Institute for Animal Husbandry, Belgrade-Zemun",
journal = "Proceedings of the 12th International Symposium Modern Trends in Livestock Production, Belgrade",
title = "Antimicrobial susceptibility testing of streptococcus suis isolates to common antibiotics used in pig farms",
pages = "148-154",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_676"
}

Stanojković, A., Radović, Č., Stanojković-Sebić, A., Gogić, M., Mandić, V., Nišavić, J.,& Petričević, M.. (2019). Antimicrobial susceptibility testing of streptococcus suis isolates to common antibiotics used in pig farms. in Proceedings of the 12th International Symposium Modern Trends in Livestock Production, Belgrade
Institute for Animal Husbandry, Belgrade-Zemun., 148-154.
https://hdl.handle.net/21.15107/rcub_ristocar_676

Stanojković A, Radović Č, Stanojković-Sebić A, Gogić M, Mandić V, Nišavić J, Petričević M. Antimicrobial susceptibility testing of streptococcus suis isolates to common antibiotics used in pig farms. in Proceedings of the 12th International Symposium Modern Trends in Livestock Production, Belgrade. 2019;:148-154.
https://hdl.handle.net/21.15107/rcub_ristocar_676 .

Stanojković, Aleksandar, Radović, Čedomir, Stanojković-Sebić, Aleksandra, Gogić, Marija, Mandić, Violeta, Nišavić, Jakov, Petričević, Maja, "Antimicrobial susceptibility testing of streptococcus suis isolates to common antibiotics used in pig farms" in Proceedings of the 12th International Symposium Modern Trends in Livestock Production, Belgrade (2019):148-154,
https://hdl.handle.net/21.15107/rcub_ristocar_676 .

TY  - JOUR
AU  - Milić, Nenad
AU  - Nišavić, Jakov
AU  - Zorić, Andrea
AU  - Krnjaić, Dejan
AU  - Radojičić, Marina
AU  - Stanojković, Aleksandar
PY  - 2017
UR  - http://r.istocar.bg.ac.rs/handle/123456789/516
AB  - Newcastle disease virus (NDV) is one of the most important viral pathogens of avian species and the causative agent of atypical fowl plague, a highly contagious and economically important disease characterized by high mortality rates and reduction of egg production. The HN and F proteins are the main targets for immune response to NDV. Vaccination of poultry with live and inactivated NDV vaccines is the most effective method of control and prevention of Newcastle disease, however due to their disadvantages, efforts are being invested into developing subunit vaccines. To this end, the NDV HN and/or F protein have been expressed using different viruses as vectors, but have also been expressed using transgenic plant systems, yeast and lactic acid bacteria in order to produce the NDV subunit vaccine. Many authors have investigated the possibility of preparation of vaccines from purified and biologically active NDV subunits with HN and F glycoproteins, purified from nucleocapsids, viral ribonucleic acid (RNA) and pyrogens. The above mentioned viral glycoproteins with preserved antigenic structure and biological activities can be used as subunit vaccinal antigens due to their immunogenic properties.
AB  - Virus Newcastle bolesti je jedan od najznačajnijih patogena u populaciji ptica i domaće živine koji izaziva atipičnu kugu živine, kontagiozno oboljenje koje prati visoka stopa morbiditeta i mortaliteta, što ima za posledicu i velike ekonomske gubitke u živinarstvu. Glikoproteinski HN i F antigeni virusa atipične kuge živine su najznačajniji prilikom razvoja imunološkog odgovora prijemčivih jedinki. Vakcinacija živine živim i inaktivisanim vakcinama protiv virusa Newcastle bolesti predstavlja najefikasniji metod kontrole i prevencije navedenog oboljenja, međutim klasične vakcine imaju izvesne nedostatke i iz tog razloga se sve više istraživanja se usmerava na razvoj subjediničnih vakcina. U cilju razvoja subjediničnih vakcina u današnje vreme se za ekspresiju HN i F proteina virusa Newcastle bolesti koriste različiti vektori kao što su virusi, transgene biljke, kvasci i mlečnokiselinske bakterije. Pored toga, mnogi autori su ispitivali mogućnosti pripremanja subjediničnih vakcina od prečišćenih i biološki aktivnih subjedinica, odnosno HN i F glikoproteina pomenutog virusa, oslobođenih od nukleokapsida sa virusnom ribonukleinskom kiselinom (RNK) i pirogena. Virusni glikoproteini sa očuvanom antigenskom strukturom i biološkim aktivnostima se zbog svojih imunogenih svojstava mogu koristiti kao subjedinični vakcinalni antigeni.
PB  - Institute for Animal Husbandry, Belgrade
T2  - Biotechnology in Animal Husbandry
T1  - Overview of current advances in the development of subunit and recombinant vaccines against Newcastle disease virus
T1  - Pregled savremenih saznanja o razvoju subjediničnih i rekombinantnih vakcina protiv virusa Newcastle bolesti živine
VL  - 33
IS  - 1
SP  - 1
EP  - 11
DO  - 10.2298/BAH1701001M
ER  - 

@article{
author = "Milić, Nenad and Nišavić, Jakov and Zorić, Andrea and Krnjaić, Dejan and Radojičić, Marina and Stanojković, Aleksandar",
year = "2017",
abstract = "Newcastle disease virus (NDV) is one of the most important viral pathogens of avian species and the causative agent of atypical fowl plague, a highly contagious and economically important disease characterized by high mortality rates and reduction of egg production. The HN and F proteins are the main targets for immune response to NDV. Vaccination of poultry with live and inactivated NDV vaccines is the most effective method of control and prevention of Newcastle disease, however due to their disadvantages, efforts are being invested into developing subunit vaccines. To this end, the NDV HN and/or F protein have been expressed using different viruses as vectors, but have also been expressed using transgenic plant systems, yeast and lactic acid bacteria in order to produce the NDV subunit vaccine. Many authors have investigated the possibility of preparation of vaccines from purified and biologically active NDV subunits with HN and F glycoproteins, purified from nucleocapsids, viral ribonucleic acid (RNA) and pyrogens. The above mentioned viral glycoproteins with preserved antigenic structure and biological activities can be used as subunit vaccinal antigens due to their immunogenic properties., Virus Newcastle bolesti je jedan od najznačajnijih patogena u populaciji ptica i domaće živine koji izaziva atipičnu kugu živine, kontagiozno oboljenje koje prati visoka stopa morbiditeta i mortaliteta, što ima za posledicu i velike ekonomske gubitke u živinarstvu. Glikoproteinski HN i F antigeni virusa atipične kuge živine su najznačajniji prilikom razvoja imunološkog odgovora prijemčivih jedinki. Vakcinacija živine živim i inaktivisanim vakcinama protiv virusa Newcastle bolesti predstavlja najefikasniji metod kontrole i prevencije navedenog oboljenja, međutim klasične vakcine imaju izvesne nedostatke i iz tog razloga se sve više istraživanja se usmerava na razvoj subjediničnih vakcina. U cilju razvoja subjediničnih vakcina u današnje vreme se za ekspresiju HN i F proteina virusa Newcastle bolesti koriste različiti vektori kao što su virusi, transgene biljke, kvasci i mlečnokiselinske bakterije. Pored toga, mnogi autori su ispitivali mogućnosti pripremanja subjediničnih vakcina od prečišćenih i biološki aktivnih subjedinica, odnosno HN i F glikoproteina pomenutog virusa, oslobođenih od nukleokapsida sa virusnom ribonukleinskom kiselinom (RNK) i pirogena. Virusni glikoproteini sa očuvanom antigenskom strukturom i biološkim aktivnostima se zbog svojih imunogenih svojstava mogu koristiti kao subjedinični vakcinalni antigeni.",
publisher = "Institute for Animal Husbandry, Belgrade",
journal = "Biotechnology in Animal Husbandry",
title = "Overview of current advances in the development of subunit and recombinant vaccines against Newcastle disease virus, Pregled savremenih saznanja o razvoju subjediničnih i rekombinantnih vakcina protiv virusa Newcastle bolesti živine",
volume = "33",
number = "1",
pages = "1-11",
doi = "10.2298/BAH1701001M"
}

Milić, N., Nišavić, J., Zorić, A., Krnjaić, D., Radojičić, M.,& Stanojković, A.. (2017). Overview of current advances in the development of subunit and recombinant vaccines against Newcastle disease virus. in Biotechnology in Animal Husbandry
Institute for Animal Husbandry, Belgrade., 33(1), 1-11.
https://doi.org/10.2298/BAH1701001M

Milić N, Nišavić J, Zorić A, Krnjaić D, Radojičić M, Stanojković A. Overview of current advances in the development of subunit and recombinant vaccines against Newcastle disease virus. in Biotechnology in Animal Husbandry. 2017;33(1):1-11.
doi:10.2298/BAH1701001M .

Milić, Nenad, Nišavić, Jakov, Zorić, Andrea, Krnjaić, Dejan, Radojičić, Marina, Stanojković, Aleksandar, "Overview of current advances in the development of subunit and recombinant vaccines against Newcastle disease virus" in Biotechnology in Animal Husbandry, 33, no. 1 (2017):1-11,
https://doi.org/10.2298/BAH1701001M . .

TY  - JOUR
AU  - Nišavić, Jakov
AU  - Milić, Nenad
AU  - Zorić, Andrea
AU  - Bojkovski, Jovan
AU  - Stanojković, Aleksandar
PY  - 2016
UR  - http://r.istocar.bg.ac.rs/handle/123456789/487
AB  - Viral infections of swine cause significant economic losses in swine husbandry. They manifest in death of infected animals of different ages or in decreased productivity during the manufacturing process. Having that in mind, rapid and reliable diagnostics of viral infections is crucial in the prevention of disease transmission in herds of swine. Today, virological laboratories all over the world use different diagnostic methods such as isolation of virus in cell lines, ELISA, virus neutralization test, direct and indirect immunofluorescence and hemagglutination and hemagglutination inhibition tests. Virus isolation, virus neutralization test and some other standard virological methods are time consuming and rather expensive, therefore, molecular methods such as conventional PCR, RT - PCR, real-time PCR and direct sequencing methods are applied worldwide as fast and reliable. Their application is especially necessary for the detection of viruses which cannot be identified by using standard virological methods.
AB  - Virusne infekcije izazivaju značajne ekonomske gubitke u svinjarskoj proizvodnji. One se ispoljavaju kako kroz pojavu uginuća životinja, tako i kroz smanjenje produktivnosti. U cilju otkrivanja i sprečavanja širenja virusnih oboljenja svinja danas se u svetu primenjuju standardne i molekularne metode virusološke dijagnostike. Od standardnih metoda dijagnostike u upotrebi su metode izolacije virusa u kulturi ćelija, zatim ELISA, direktna i indirektna imunofluorescencija, kao i hemaglutinacija i inhibicija hemaglutinacije. Primena navedenih metoda podrazumeva duže vreme potrebno za dobijanje rezultata ispitivanja od najmanje 5 do 7 dana. Međutim, primena savremenih molekularnih metoda virusološke dijagnostike kao što su PCR, real-time PCR, odnosno metoda direktnog sekvenciranja, podrazumeva kraće vreme potrebno za dobijanje rezultata, odnosno omogućava preciznu dijagnostiku oboljenja u kraćem vremenskom periodu. Pored ovoga, značaj primene ovih metoda se ogleda i u otkrivanju virusa čije se prisustvo u uzorcima na drugi način, odnosno primenom standardnih metoda virusološke dijagnostike ne može detektovati.
PB  - Institute for Animal Husbandry, Belgrade
T2  - Biotechnology in Animal Husbandry
T1  - The application of PCR based methods in diagnostics of some viral infections of swine
T1  - Primena molekularnih metoda zasnovanih na lančanoj reakciji polimeraze u dijagnostici nekih infekcija svinja
VL  - 32
IS  - 4
SP  - 321
EP  - 329
DO  - 10.2298/bah1604321N
ER  - 

@article{
author = "Nišavić, Jakov and Milić, Nenad and Zorić, Andrea and Bojkovski, Jovan and Stanojković, Aleksandar",
year = "2016",
abstract = "Viral infections of swine cause significant economic losses in swine husbandry. They manifest in death of infected animals of different ages or in decreased productivity during the manufacturing process. Having that in mind, rapid and reliable diagnostics of viral infections is crucial in the prevention of disease transmission in herds of swine. Today, virological laboratories all over the world use different diagnostic methods such as isolation of virus in cell lines, ELISA, virus neutralization test, direct and indirect immunofluorescence and hemagglutination and hemagglutination inhibition tests. Virus isolation, virus neutralization test and some other standard virological methods are time consuming and rather expensive, therefore, molecular methods such as conventional PCR, RT - PCR, real-time PCR and direct sequencing methods are applied worldwide as fast and reliable. Their application is especially necessary for the detection of viruses which cannot be identified by using standard virological methods., Virusne infekcije izazivaju značajne ekonomske gubitke u svinjarskoj proizvodnji. One se ispoljavaju kako kroz pojavu uginuća životinja, tako i kroz smanjenje produktivnosti. U cilju otkrivanja i sprečavanja širenja virusnih oboljenja svinja danas se u svetu primenjuju standardne i molekularne metode virusološke dijagnostike. Od standardnih metoda dijagnostike u upotrebi su metode izolacije virusa u kulturi ćelija, zatim ELISA, direktna i indirektna imunofluorescencija, kao i hemaglutinacija i inhibicija hemaglutinacije. Primena navedenih metoda podrazumeva duže vreme potrebno za dobijanje rezultata ispitivanja od najmanje 5 do 7 dana. Međutim, primena savremenih molekularnih metoda virusološke dijagnostike kao što su PCR, real-time PCR, odnosno metoda direktnog sekvenciranja, podrazumeva kraće vreme potrebno za dobijanje rezultata, odnosno omogućava preciznu dijagnostiku oboljenja u kraćem vremenskom periodu. Pored ovoga, značaj primene ovih metoda se ogleda i u otkrivanju virusa čije se prisustvo u uzorcima na drugi način, odnosno primenom standardnih metoda virusološke dijagnostike ne može detektovati.",
publisher = "Institute for Animal Husbandry, Belgrade",
journal = "Biotechnology in Animal Husbandry",
title = "The application of PCR based methods in diagnostics of some viral infections of swine, Primena molekularnih metoda zasnovanih na lančanoj reakciji polimeraze u dijagnostici nekih infekcija svinja",
volume = "32",
number = "4",
pages = "321-329",
doi = "10.2298/bah1604321N"
}

Nišavić, J., Milić, N., Zorić, A., Bojkovski, J.,& Stanojković, A.. (2016). The application of PCR based methods in diagnostics of some viral infections of swine. in Biotechnology in Animal Husbandry
Institute for Animal Husbandry, Belgrade., 32(4), 321-329.
https://doi.org/10.2298/bah1604321N

Nišavić J, Milić N, Zorić A, Bojkovski J, Stanojković A. The application of PCR based methods in diagnostics of some viral infections of swine. in Biotechnology in Animal Husbandry. 2016;32(4):321-329.
doi:10.2298/bah1604321N .

Nišavić, Jakov, Milić, Nenad, Zorić, Andrea, Bojkovski, Jovan, Stanojković, Aleksandar, "The application of PCR based methods in diagnostics of some viral infections of swine" in Biotechnology in Animal Husbandry, 32, no. 4 (2016):321-329,
https://doi.org/10.2298/bah1604321N . .