Mandić, Violeta

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Effect of pH on germination and seedling growth of maize.

Mandić, Violeta; Krnjaja, vesna; Simić, Aleksandar; Petričević, Maja; Gogić, Marija; Brankov, Milan; Stanojković, Aleksandar

(Institute for Animal Husbandry, Belgrade-Zemun, 2023-12)

TY  - JOUR
AU  - Mandić, Violeta
AU  - Krnjaja, vesna
AU  - Simić, Aleksandar
AU  - Petričević, Maja
AU  - Gogić, Marija
AU  - Brankov, Milan
AU  - Stanojković, Aleksandar
PY  - 2023-12
UR  - http://r.istocar.bg.ac.rs/handle/123456789/971
AB  - Soil pH is one of the most important limiting factors for crop cultivation, including maize. About 30-40% of the world's arable land is acidic, and over a billion ha is alkaline. Today, there are 205 million ha of arable land under maize in the world, so it ranks third in area after rice and wheat. Maize hybrids have wide genetic variability and high pH tolerance. We tested the tolerance of maize hybrids seedlings of ZP 4708 and ZP 5797 to pH 5, 6, 7, and 8. Results showed that hybrid ZP 4708 has a higher adaptability to low and high pH, due to the higher value of germination energy (GE), shoot length (ShL), shoot fresh weight (ShFW), shoot dry weight (ShDW), germination rate index (GRI), and seedling vigor index (SVI) than hybrid ZP 5797. Results further showed, on average for hybrids, that pH 5 and pH 8 significantly decreased the GE, root length (RL), ShL, root fresh weight (RFW), ShFW, root dry weight (RDW), shoot fresh weight (ShDW), and SVI. These parameters did not differ significantly between pH 6 and pH 7. Seed germination was not affected by the pH, indicating that the seed vigor of both hybrids supports full germination potential. However, it could be expected that exposure to the lowest and highest pH values of the medium could affect seedlings growth in the field conditions, even though germination is high. SVI showed a positive and significant correlation with all investigated parameters, except germination (G) and relative seedling water content (RSWC).
AB  - pH zemlji ta je jedan od va nih ograni avajućih faktora za gajenje useva, a samim tim i kukuruza. Oko 30-40% svetskih obradivih povr ina je kisele reakcije, a preko milijardu ha je alkalno. Danas je u svetu 205 miliona ha obradivih povr ina pod kukuruzom, tako da on zauzima treće mesto po povr ini posle pirin a i p enice. Hibridi kukuruza imaju iroku genetsku varijabilnost i toleranciju za pH. Ispitivali smo toleranciju klijanaca novih hibrida kukuruza ZP 4708 i ZP 5797 na pH 5, 6, 7 i 8. Rezultati su pokazali da hibrid ZP 4 08 ima bolju sposobnost adaptacije na nisku i visoku pH vrednost jer je imao veću energiju klijanja (EK), du inu stabla (DS), sve u masu stabla (SvMS), suvu masu stabla (SuMS), indeks klijavosti (IK) i vigor indeks (VI) od hibrida ZP 5797. Rezultati su dalje pokazali, u proseku za hibride, da su niski (pH 5) i visoki pH nivo medijuma (pH8) zna ajno smanjili EK, du inu korena (DK), DS, sve u masu korena (SvMK), SvMS, suvu masu korena (SuMK), SuMS i VI. Ovi parametri se nisu razlikovali između tretmana pH 6 i pH . pH medijuma nije uticala na klijanje semena to ukazuje da snaga semena oba hibrida podr ava pun potencijal klijanja. Međutim, izlaganje najni im i najvi im pH vrednostima medijuma je dovelo do toga da nije moguće o ekivati jake klijance, iako je klijavost visoka. VI je pokazao pozitivnu i zna ajnu korelaciju sa svim ispitivanim parametrima, osim sa klijanjem semena i relativnim sadr ajem vode u klijancima.
PB  - Institute for Animal Husbandry, Belgrade-Zemun
T2  - Biotechnology in Animal Husbandry
T1  - Effect of pH on germination and seedling growth of maize.
T1  - Uticaj pH vrednosti na klijanje i rast klijanaca kukuruza
VL  - 39
IS  - 2
SP  - 195
EP  - 203
DO  - 10.2298/BAH2302195M
ER  - 
@article{
author = "Mandić, Violeta and Krnjaja, vesna and Simić, Aleksandar and Petričević, Maja and Gogić, Marija and Brankov, Milan and Stanojković, Aleksandar",
year = "2023-12",
abstract = "Soil pH is one of the most important limiting factors for crop cultivation, including maize. About 30-40% of the world's arable land is acidic, and over a billion ha is alkaline. Today, there are 205 million ha of arable land under maize in the world, so it ranks third in area after rice and wheat. Maize hybrids have wide genetic variability and high pH tolerance. We tested the tolerance of maize hybrids seedlings of ZP 4708 and ZP 5797 to pH 5, 6, 7, and 8. Results showed that hybrid ZP 4708 has a higher adaptability to low and high pH, due to the higher value of germination energy (GE), shoot length (ShL), shoot fresh weight (ShFW), shoot dry weight (ShDW), germination rate index (GRI), and seedling vigor index (SVI) than hybrid ZP 5797. Results further showed, on average for hybrids, that pH 5 and pH 8 significantly decreased the GE, root length (RL), ShL, root fresh weight (RFW), ShFW, root dry weight (RDW), shoot fresh weight (ShDW), and SVI. These parameters did not differ significantly between pH 6 and pH 7. Seed germination was not affected by the pH, indicating that the seed vigor of both hybrids supports full germination potential. However, it could be expected that exposure to the lowest and highest pH values of the medium could affect seedlings growth in the field conditions, even though germination is high. SVI showed a positive and significant correlation with all investigated parameters, except germination (G) and relative seedling water content (RSWC)., pH zemlji ta je jedan od va nih ograni avajućih faktora za gajenje useva, a samim tim i kukuruza. Oko 30-40% svetskih obradivih povr ina je kisele reakcije, a preko milijardu ha je alkalno. Danas je u svetu 205 miliona ha obradivih povr ina pod kukuruzom, tako da on zauzima treće mesto po povr ini posle pirin a i p enice. Hibridi kukuruza imaju iroku genetsku varijabilnost i toleranciju za pH. Ispitivali smo toleranciju klijanaca novih hibrida kukuruza ZP 4708 i ZP 5797 na pH 5, 6, 7 i 8. Rezultati su pokazali da hibrid ZP 4 08 ima bolju sposobnost adaptacije na nisku i visoku pH vrednost jer je imao veću energiju klijanja (EK), du inu stabla (DS), sve u masu stabla (SvMS), suvu masu stabla (SuMS), indeks klijavosti (IK) i vigor indeks (VI) od hibrida ZP 5797. Rezultati su dalje pokazali, u proseku za hibride, da su niski (pH 5) i visoki pH nivo medijuma (pH8) zna ajno smanjili EK, du inu korena (DK), DS, sve u masu korena (SvMK), SvMS, suvu masu korena (SuMK), SuMS i VI. Ovi parametri se nisu razlikovali između tretmana pH 6 i pH . pH medijuma nije uticala na klijanje semena to ukazuje da snaga semena oba hibrida podr ava pun potencijal klijanja. Međutim, izlaganje najni im i najvi im pH vrednostima medijuma je dovelo do toga da nije moguće o ekivati jake klijance, iako je klijavost visoka. VI je pokazao pozitivnu i zna ajnu korelaciju sa svim ispitivanim parametrima, osim sa klijanjem semena i relativnim sadr ajem vode u klijancima.",
publisher = "Institute for Animal Husbandry, Belgrade-Zemun",
journal = "Biotechnology in Animal Husbandry",
title = "Effect of pH on germination and seedling growth of maize., Uticaj pH vrednosti na klijanje i rast klijanaca kukuruza",
volume = "39",
number = "2",
pages = "195-203",
doi = "10.2298/BAH2302195M"
}
Mandić, V., Krnjaja, v., Simić, A., Petričević, M., Gogić, M., Brankov, M.,& Stanojković, A.. (2023-12). Effect of pH on germination and seedling growth of maize.. in Biotechnology in Animal Husbandry
Institute for Animal Husbandry, Belgrade-Zemun., 39(2), 195-203.
https://doi.org/10.2298/BAH2302195M
Mandić V, Krnjaja V, Simić A, Petričević M, Gogić M, Brankov M, Stanojković A. Effect of pH on germination and seedling growth of maize.. in Biotechnology in Animal Husbandry. 2023;39(2):195-203.
doi:10.2298/BAH2302195M .
Mandić, Violeta, Krnjaja, vesna, Simić, Aleksandar, Petričević, Maja, Gogić, Marija, Brankov, Milan, Stanojković, Aleksandar, "Effect of pH on germination and seedling growth of maize." in Biotechnology in Animal Husbandry, 39, no. 2 (2023-12):195-203,
https://doi.org/10.2298/BAH2302195M . .

STREPTOCOCCUS SUIS, TWO-FACED GAME CHANGER

Stanojković, Aleksandar; Stanišić, Nikola; Delić, Nikola; Bošnjak, Ivan; Mandić, Violeta; Stanojković-Sebić, Aleksandra; Nišavić, Jakov

(Institute for Animal Husbandry, Belgrade - Zemun, 2023-10-04)

TY  - CONF
AU  - Stanojković, Aleksandar
AU  - Stanišić, Nikola
AU  - Delić, Nikola
AU  - Bošnjak, Ivan
AU  - Mandić, Violeta
AU  - Stanojković-Sebić, Aleksandra
AU  - Nišavić, Jakov
PY  - 2023-10-04
UR  - http://r.istocar.bg.ac.rs/handle/123456789/896
AB  - Streptococcus suis infection is one of the major health problems in the
swine industry worldwide. During the last decade, the number of reported human
cases due to S. suis has dramatically increased, and while most sporadic human
cases of infection appear to be due to close occupational contact with pigs/pork
products. S. suis infection is considered to be multifactorial, with transition from
subclinical to clinical that depends on many factors. These factors can be divided in
two groups, host-based and external factors. Pathogenesis of S. suis infection can
be divided into 4 phases: adherence to and colonisation of mucosal and epithelial
surfaces, invasion into deeper tissues and entering the bloodstream, crossing bloodbrain
barrier and inflammation. S. suis virulence-associated factors are divided into
the following 4 groups: surface/secreted elements, enzymes (such as including
proteases), transcription factors and regulatory systems and other factors (such as
transporting and secreting systems). Therefore significant research support is needed
to obtain a vaccine as a valuable and universal protection against disease caused by S.
suis strains and thus national and international support will be crucial for the aim many
researchers hope for.
PB  - Institute for Animal Husbandry, Belgrade - Zemun
T1  - STREPTOCOCCUS SUIS, TWO-FACED GAME CHANGER
SP  - 251
EP  - 266
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_896
ER  - 
@conference{
author = "Stanojković, Aleksandar and Stanišić, Nikola and Delić, Nikola and Bošnjak, Ivan and Mandić, Violeta and Stanojković-Sebić, Aleksandra and Nišavić, Jakov",
year = "2023-10-04",
abstract = "Streptococcus suis infection is one of the major health problems in the
swine industry worldwide. During the last decade, the number of reported human
cases due to S. suis has dramatically increased, and while most sporadic human
cases of infection appear to be due to close occupational contact with pigs/pork
products. S. suis infection is considered to be multifactorial, with transition from
subclinical to clinical that depends on many factors. These factors can be divided in
two groups, host-based and external factors. Pathogenesis of S. suis infection can
be divided into 4 phases: adherence to and colonisation of mucosal and epithelial
surfaces, invasion into deeper tissues and entering the bloodstream, crossing bloodbrain
barrier and inflammation. S. suis virulence-associated factors are divided into
the following 4 groups: surface/secreted elements, enzymes (such as including
proteases), transcription factors and regulatory systems and other factors (such as
transporting and secreting systems). Therefore significant research support is needed
to obtain a vaccine as a valuable and universal protection against disease caused by S.
suis strains and thus national and international support will be crucial for the aim many
researchers hope for.",
publisher = "Institute for Animal Husbandry, Belgrade - Zemun",
title = "STREPTOCOCCUS SUIS, TWO-FACED GAME CHANGER",
pages = "251-266",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_896"
}
Stanojković, A., Stanišić, N., Delić, N., Bošnjak, I., Mandić, V., Stanojković-Sebić, A.,& Nišavić, J.. (2023-10-04). STREPTOCOCCUS SUIS, TWO-FACED GAME CHANGER. 
Institute for Animal Husbandry, Belgrade - Zemun., 251-266.
https://hdl.handle.net/21.15107/rcub_ristocar_896
Stanojković A, Stanišić N, Delić N, Bošnjak I, Mandić V, Stanojković-Sebić A, Nišavić J. STREPTOCOCCUS SUIS, TWO-FACED GAME CHANGER. 2023;:251-266.
https://hdl.handle.net/21.15107/rcub_ristocar_896 .
Stanojković, Aleksandar, Stanišić, Nikola, Delić, Nikola, Bošnjak, Ivan, Mandić, Violeta, Stanojković-Sebić, Aleksandra, Nišavić, Jakov, "STREPTOCOCCUS SUIS, TWO-FACED GAME CHANGER" (2023-10-04):251-266,
https://hdl.handle.net/21.15107/rcub_ristocar_896 .

Phosphates as food additives in meat products manufactured in republic of Serbia

Petričević, Maja; Stamenić, Tamara; Keškić, Tanja; Stanišić, Nikola; Petričević, Veselin; Mandić, Violeta; Pisinov, Boris

(Institute for Animal Husbandry, Belgrade-Zemun, 2023-10)

TY  - CONF
AU  - Petričević, Maja
AU  - Stamenić, Tamara
AU  - Keškić, Tanja
AU  - Stanišić, Nikola
AU  - Petričević, Veselin
AU  - Mandić, Violeta
AU  - Pisinov, Boris
PY  - 2023-10
UR  - http://r.istocar.bg.ac.rs/handle/123456789/892
AB  - The widespread use of food additives has experienced a remarkable increase in recent years.
Within the realm of processed meat and meat products, the indispensability of phosphates as additives is
evident, as they significantly enhance the functionality of meat proteins. Furthermore, their application in
meat product manufacturing offers a plethora of advantages, encompassing pH modification and
stabilization, augmented water holding capacity leading to heightened yields, diminished weight loss during
cooking, and an overall enhancement in texture and sensory attributes such as tenderness, juiciness, color,
and flavor. Notably, they serve as a valuable source of essential phosphorus for consumers, crucial for the
growth, maintenance, and repair of all living organisms' tissues and cells. The Rulebook ("Official Gazette of
RS", No. 50/2019 and 34/2023) of the Republic of Serbia establishes the acceptable phosphate levels in
various meat products, stipulating that the amount in cooked sausages should not exceed 8.0 g/kg. This
study aims to examine the phosphate content in meat products available in the Republic of Serbia's market. It
seeks to determine whether producers accurately incorporate additives into their products. Over a period of
two years, the phosphate content in meat products was monitored in the group of cooked sausages within the
subgroups of finely and coarsely ground cooked sausages. Over a span of two years, the phosphate content
was meticulously monitored selected cooked sausages, both finely and coarsely ground in three lot years
resulting in 156 samples. The study examined 52 meat products available on the market were examined: 37
finely ground and 15 coarsely ground cooked sausages. The phosphate content in the products varied greatly
and ranged from 2.01g/kg - 6.98g/kg for finely ground sausages, while the phosphate content for coarsely
ground sausages ranged from 3.33g/kg - 9.15g/kg. The phosphate content was higher than the maximum
allowed concentration in only one sample of one lot series of the single coarsely ground sausage (9.15g/kg).
Based on the comprehensive market survey, it can be confidently concluded that producers appropriately
employ phosphates in their products, despite the diversity in scope and technology involved in meat product
preparation.
PB  - Institute for Animal Husbandry, Belgrade-Zemun
C3  - Book of abstracts of the 14th International Symposium „Modern trends in livestock production“
T1  - Phosphates as food additives in meat products manufactured in republic of Serbia
SP  - 72
EP  - 72
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_892
ER  - 
@conference{
author = "Petričević, Maja and Stamenić, Tamara and Keškić, Tanja and Stanišić, Nikola and Petričević, Veselin and Mandić, Violeta and Pisinov, Boris",
year = "2023-10",
abstract = "The widespread use of food additives has experienced a remarkable increase in recent years.
Within the realm of processed meat and meat products, the indispensability of phosphates as additives is
evident, as they significantly enhance the functionality of meat proteins. Furthermore, their application in
meat product manufacturing offers a plethora of advantages, encompassing pH modification and
stabilization, augmented water holding capacity leading to heightened yields, diminished weight loss during
cooking, and an overall enhancement in texture and sensory attributes such as tenderness, juiciness, color,
and flavor. Notably, they serve as a valuable source of essential phosphorus for consumers, crucial for the
growth, maintenance, and repair of all living organisms' tissues and cells. The Rulebook ("Official Gazette of
RS", No. 50/2019 and 34/2023) of the Republic of Serbia establishes the acceptable phosphate levels in
various meat products, stipulating that the amount in cooked sausages should not exceed 8.0 g/kg. This
study aims to examine the phosphate content in meat products available in the Republic of Serbia's market. It
seeks to determine whether producers accurately incorporate additives into their products. Over a period of
two years, the phosphate content in meat products was monitored in the group of cooked sausages within the
subgroups of finely and coarsely ground cooked sausages. Over a span of two years, the phosphate content
was meticulously monitored selected cooked sausages, both finely and coarsely ground in three lot years
resulting in 156 samples. The study examined 52 meat products available on the market were examined: 37
finely ground and 15 coarsely ground cooked sausages. The phosphate content in the products varied greatly
and ranged from 2.01g/kg - 6.98g/kg for finely ground sausages, while the phosphate content for coarsely
ground sausages ranged from 3.33g/kg - 9.15g/kg. The phosphate content was higher than the maximum
allowed concentration in only one sample of one lot series of the single coarsely ground sausage (9.15g/kg).
Based on the comprehensive market survey, it can be confidently concluded that producers appropriately
employ phosphates in their products, despite the diversity in scope and technology involved in meat product
preparation.",
publisher = "Institute for Animal Husbandry, Belgrade-Zemun",
journal = "Book of abstracts of the 14th International Symposium „Modern trends in livestock production“",
title = "Phosphates as food additives in meat products manufactured in republic of Serbia",
pages = "72-72",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_892"
}
Petričević, M., Stamenić, T., Keškić, T., Stanišić, N., Petričević, V., Mandić, V.,& Pisinov, B.. (2023-10). Phosphates as food additives in meat products manufactured in republic of Serbia. in Book of abstracts of the 14th International Symposium „Modern trends in livestock production“
Institute for Animal Husbandry, Belgrade-Zemun., 72-72.
https://hdl.handle.net/21.15107/rcub_ristocar_892
Petričević M, Stamenić T, Keškić T, Stanišić N, Petričević V, Mandić V, Pisinov B. Phosphates as food additives in meat products manufactured in republic of Serbia. in Book of abstracts of the 14th International Symposium „Modern trends in livestock production“. 2023;:72-72.
https://hdl.handle.net/21.15107/rcub_ristocar_892 .
Petričević, Maja, Stamenić, Tamara, Keškić, Tanja, Stanišić, Nikola, Petričević, Veselin, Mandić, Violeta, Pisinov, Boris, "Phosphates as food additives in meat products manufactured in republic of Serbia" in Book of abstracts of the 14th International Symposium „Modern trends in livestock production“ (2023-10):72-72,
https://hdl.handle.net/21.15107/rcub_ristocar_892 .

Aflatoxin and fumonisin contamination of maize grains harvested during 2018–2022 in Serbia

Krnjaja, Vesna; Stanković, Slavica; Obradović, Ana; Mandić, Violeta; Petrović, Tanja; Stanišić, Nikola; Delić, Nikola

(Institute for Animal Husbandry, Belgrade-Zemun, Serbia, 2023)

TY  - CONF
AU  - Krnjaja, Vesna
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Mandić, Violeta
AU  - Petrović, Tanja
AU  - Stanišić, Nikola
AU  - Delić, Nikola
PY  - 2023
UR  - http://r.istocar.bg.ac.rs/handle/123456789/904
AB  - Maize is a staple food worldwide, used in human nutrition and as the main component of livestock 
feed. It contains nutrients such as carbohydrates (sugars and starch), proteins, fibres, minerals and vitamins 
and has the highest polysaccharide starch content (more than 70%). Maize grains are a suitable substrate for 
fungal infection and are frequently contaminated by the species from the Aspergillus and Fusarium
genera. These fungi produce toxic secondary metabolites named mycotoxins. Aflatoxins (AFs) and type-B 
fumonisins (FBs) are the most detected mycotoxins in maize grains. Maize intoxication with AFs and FBs 
can lead to human and animal health disorders, causing acute and chronic mycotoxicosis. Aflatoxins 
produced by Aspergillus spp. have immunosuppressive, carcinogenic, and hepatotoxic properties and induce 
growth impairments. Fumonisins produced primarily by Fusarium spp. have neurotoxic, nephrotoxic, and 
hepatotoxic effects in animals and are classified as potentially carcinogenic in humans. The co-occurrence of 
AFs and FBs in maize has synergistic toxicological effects and poses a greater risk to human and animal 
health. The natural occurrence AFs and FBs in 65 maize grain samples collected during harvest in 2018 (13 
samples), 2019 (11 samples), 2020 (9 samples), 2021 (14 samples) and 2022 (18 samples) was evaluated 
using the enzyme-linked immune sorbent assay (ELISA). The average levels of AFs and FBs in mycotoxin positive samples were 3.02 and 2910 µg kg–1 (2018), 5.28 and 2710 µg kg–1 (2019), 2.35 and 10980 µg kg–1
(2020), 6.81 and 4950 µg kg–1 (2021) and 5.32 and 20310 µg kg–1 (2022), respectively. The co-occurrence of 
AFs and FBs was detected in 23.08% (2018), 18.18% (2019), 22.22% (2020), 64.29% (2021) and 22.22% 
(2022) of maize samples. The maximum limits of 10 µg kg–1 for AFs in maize and 4000 µg kg–1 for FBs in 
unprocessed maize, prescribed by regulations of the European Union and Serbia, were exceeded for AFs in 
14.29% (2021) and 5.56% (2022) of tested maize samples and for FBs in 7.69% (2018), 66.67% (2020), 
28.57% (2021) and 41.67% (2022) tested maize samples. These results indicate the need for continuous 
monitoring of the health status of harvested maize grains and risk assessment of the potential presence of 
mycotoxins in the food chain to avoid adverse effects on human and animal health.
PB  - Institute for Animal Husbandry, Belgrade-Zemun, Serbia
C3  - Book of Abstracts of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia.
T1  - Aflatoxin and fumonisin contamination of maize grains harvested during 2018–2022 in Serbia
SP  - 79
EP  - 79
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_904
ER  - 
@conference{
author = "Krnjaja, Vesna and Stanković, Slavica and Obradović, Ana and Mandić, Violeta and Petrović, Tanja and Stanišić, Nikola and Delić, Nikola",
year = "2023",
abstract = "Maize is a staple food worldwide, used in human nutrition and as the main component of livestock 
feed. It contains nutrients such as carbohydrates (sugars and starch), proteins, fibres, minerals and vitamins 
and has the highest polysaccharide starch content (more than 70%). Maize grains are a suitable substrate for 
fungal infection and are frequently contaminated by the species from the Aspergillus and Fusarium
genera. These fungi produce toxic secondary metabolites named mycotoxins. Aflatoxins (AFs) and type-B 
fumonisins (FBs) are the most detected mycotoxins in maize grains. Maize intoxication with AFs and FBs 
can lead to human and animal health disorders, causing acute and chronic mycotoxicosis. Aflatoxins 
produced by Aspergillus spp. have immunosuppressive, carcinogenic, and hepatotoxic properties and induce 
growth impairments. Fumonisins produced primarily by Fusarium spp. have neurotoxic, nephrotoxic, and 
hepatotoxic effects in animals and are classified as potentially carcinogenic in humans. The co-occurrence of 
AFs and FBs in maize has synergistic toxicological effects and poses a greater risk to human and animal 
health. The natural occurrence AFs and FBs in 65 maize grain samples collected during harvest in 2018 (13 
samples), 2019 (11 samples), 2020 (9 samples), 2021 (14 samples) and 2022 (18 samples) was evaluated 
using the enzyme-linked immune sorbent assay (ELISA). The average levels of AFs and FBs in mycotoxin positive samples were 3.02 and 2910 µg kg–1 (2018), 5.28 and 2710 µg kg–1 (2019), 2.35 and 10980 µg kg–1
(2020), 6.81 and 4950 µg kg–1 (2021) and 5.32 and 20310 µg kg–1 (2022), respectively. The co-occurrence of 
AFs and FBs was detected in 23.08% (2018), 18.18% (2019), 22.22% (2020), 64.29% (2021) and 22.22% 
(2022) of maize samples. The maximum limits of 10 µg kg–1 for AFs in maize and 4000 µg kg–1 for FBs in 
unprocessed maize, prescribed by regulations of the European Union and Serbia, were exceeded for AFs in 
14.29% (2021) and 5.56% (2022) of tested maize samples and for FBs in 7.69% (2018), 66.67% (2020), 
28.57% (2021) and 41.67% (2022) tested maize samples. These results indicate the need for continuous 
monitoring of the health status of harvested maize grains and risk assessment of the potential presence of 
mycotoxins in the food chain to avoid adverse effects on human and animal health.",
publisher = "Institute for Animal Husbandry, Belgrade-Zemun, Serbia",
journal = "Book of Abstracts of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia.",
title = "Aflatoxin and fumonisin contamination of maize grains harvested during 2018–2022 in Serbia",
pages = "79-79",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_904"
}
Krnjaja, V., Stanković, S., Obradović, A., Mandić, V., Petrović, T., Stanišić, N.,& Delić, N.. (2023). Aflatoxin and fumonisin contamination of maize grains harvested during 2018–2022 in Serbia. in Book of Abstracts of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia.
Institute for Animal Husbandry, Belgrade-Zemun, Serbia., 79-79.
https://hdl.handle.net/21.15107/rcub_ristocar_904
Krnjaja V, Stanković S, Obradović A, Mandić V, Petrović T, Stanišić N, Delić N. Aflatoxin and fumonisin contamination of maize grains harvested during 2018–2022 in Serbia. in Book of Abstracts of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia.. 2023;:79-79.
https://hdl.handle.net/21.15107/rcub_ristocar_904 .
Krnjaja, Vesna, Stanković, Slavica, Obradović, Ana, Mandić, Violeta, Petrović, Tanja, Stanišić, Nikola, Delić, Nikola, "Aflatoxin and fumonisin contamination of maize grains harvested during 2018–2022 in Serbia" in Book of Abstracts of the 14th International Symposium „Modern Trends in Livestock Production“, 4 – 6 October 2023, Belgrade, Serbia. (2023):79-79,
https://hdl.handle.net/21.15107/rcub_ristocar_904 .

Утицај сорте и соног стреса на почетни пораст црвене детелине

Mandić, Violeta; Bijelić, Zorica; Krnjaja, Vesna

(Друштво за крмно биље Републике Србије, 2019)

TY  - CONF
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
AU  - Krnjaja, Vesna
PY  - 2019
UR  - http://r.istocar.bg.ac.rs/handle/123456789/615
AB  - Увод: Од укупног пољопривредног земљишта у Србији око 4,6% су слана и алкална земљишта. Из тог разлога је непходно пратити толерантност биљних врста према соном стресу. Циљ овог истраживања је био да  се испита ефекат соног стреса на клијање и параметре клијанаца две домаће сорте црвене детелине.
Материјал и метод рада: Огледи су спроведени у у лабораторији Института за сточарство 2017. године. Анализиране су две домаће сорте црвене детелине К-17 и К-39. Семена су стерилисана 2% NaOCl, темељно испрана пет пута млазом дестиловане воде, осушена, помешана у авану са кварцним песком и протрљана ради механичког повређивања семењаче. У стерилисаним пластичним судовима постављено је по сто семена на филтер папиру предходно навлаженом са 10 мл одговарајуће концентрације соли (0, 50, 100, 150 и 200 mM NaCl) у 4 понављања. Судови су стављени у мрак на температури 20±1°C. Након 4 дана утврђена је енергија клијања, а након 7 дана клијавост. Дужина корена и хипокотила мерени су након десет дана на 30 насумично одабраних клијанаца у сваком третману и репликацији. Вигор индекс (ВИ) је израчунат: ВИ (%) = (дужина корена + дужина хипокотила) ×
% клијавости. Експеримент је постављен по рандомизираном блок систему. Добијени подаци су статистички обрађени методом анализе варијансе у програму Статистика 10. Такијев тест је коришћен за поређење средњих вредности на нивоу значајности p≤0.05.
Резултати: Сорта К-17 имала је већу енергију клијања, клијавост и вигор индекс него сорта К-32. Вредности испитиваних параметара се нису разликовале између 0 и 50 mM NaCl. Даље повећање концентрације соли у подлози за наклијавање (≥ 100 mM NaCl) статистички је значајно смањило вредности истраживаних параметара.
Закључак: Испитиване комерцијалне сорте црвене детелине показују осељивост на високу концентрацију соли, иако сорта К-17 има већу енергију клијања, клијавост и вигор индекс него сорта К-32.
PB  - Друштво за крмно биље Републике Србије
C3  - ЗБОРНИК АПСТРАКАТА  XIV СИМПОЗИЈУМА О КРМНОМ БИЉУ СРБИЈЕ Значај и улога крмних биљака у одрживој пољопривреди Србије
T1  - Утицај сорте и соног стреса на почетни пораст црвене детелине
SP  - 47
EP  - 48
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_615
ER  - 
@conference{
author = "Mandić, Violeta and Bijelić, Zorica and Krnjaja, Vesna",
year = "2019",
abstract = "Увод: Од укупног пољопривредног земљишта у Србији око 4,6% су слана и алкална земљишта. Из тог разлога је непходно пратити толерантност биљних врста према соном стресу. Циљ овог истраживања је био да  се испита ефекат соног стреса на клијање и параметре клијанаца две домаће сорте црвене детелине.
Материјал и метод рада: Огледи су спроведени у у лабораторији Института за сточарство 2017. године. Анализиране су две домаће сорте црвене детелине К-17 и К-39. Семена су стерилисана 2% NaOCl, темељно испрана пет пута млазом дестиловане воде, осушена, помешана у авану са кварцним песком и протрљана ради механичког повређивања семењаче. У стерилисаним пластичним судовима постављено је по сто семена на филтер папиру предходно навлаженом са 10 мл одговарајуће концентрације соли (0, 50, 100, 150 и 200 mM NaCl) у 4 понављања. Судови су стављени у мрак на температури 20±1°C. Након 4 дана утврђена је енергија клијања, а након 7 дана клијавост. Дужина корена и хипокотила мерени су након десет дана на 30 насумично одабраних клијанаца у сваком третману и репликацији. Вигор индекс (ВИ) је израчунат: ВИ (%) = (дужина корена + дужина хипокотила) ×
% клијавости. Експеримент је постављен по рандомизираном блок систему. Добијени подаци су статистички обрађени методом анализе варијансе у програму Статистика 10. Такијев тест је коришћен за поређење средњих вредности на нивоу значајности p≤0.05.
Резултати: Сорта К-17 имала је већу енергију клијања, клијавост и вигор индекс него сорта К-32. Вредности испитиваних параметара се нису разликовале између 0 и 50 mM NaCl. Даље повећање концентрације соли у подлози за наклијавање (≥ 100 mM NaCl) статистички је значајно смањило вредности истраживаних параметара.
Закључак: Испитиване комерцијалне сорте црвене детелине показују осељивост на високу концентрацију соли, иако сорта К-17 има већу енергију клијања, клијавост и вигор индекс него сорта К-32.",
publisher = "Друштво за крмно биље Републике Србије",
journal = "ЗБОРНИК АПСТРАКАТА  XIV СИМПОЗИЈУМА О КРМНОМ БИЉУ СРБИЈЕ Значај и улога крмних биљака у одрживој пољопривреди Србије",
title = "Утицај сорте и соног стреса на почетни пораст црвене детелине",
pages = "47-48",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_615"
}
Mandić, V., Bijelić, Z.,& Krnjaja, V.. (2019). Утицај сорте и соног стреса на почетни пораст црвене детелине. in ЗБОРНИК АПСТРАКАТА  XIV СИМПОЗИЈУМА О КРМНОМ БИЉУ СРБИЈЕ Значај и улога крмних биљака у одрживој пољопривреди Србије
Друштво за крмно биље Републике Србије., 47-48.
https://hdl.handle.net/21.15107/rcub_ristocar_615
Mandić V, Bijelić Z, Krnjaja V. Утицај сорте и соног стреса на почетни пораст црвене детелине. in ЗБОРНИК АПСТРАКАТА  XIV СИМПОЗИЈУМА О КРМНОМ БИЉУ СРБИЈЕ Значај и улога крмних биљака у одрживој пољопривреди Србије. 2019;:47-48.
https://hdl.handle.net/21.15107/rcub_ristocar_615 .
Mandić, Violeta, Bijelić, Zorica, Krnjaja, Vesna, "Утицај сорте и соног стреса на почетни пораст црвене детелине" in ЗБОРНИК АПСТРАКАТА  XIV СИМПОЗИЈУМА О КРМНОМ БИЉУ СРБИЈЕ Значај и улога крмних биљака у одрживој пољопривреди Србије (2019):47-48,
https://hdl.handle.net/21.15107/rcub_ristocar_615 .