TY  - JOUR
AU  - Milošević-Stanković, Ivana
AU  - Hristov, Slavča
AU  - Maksimović, Nevena
AU  - Popović, Blaženka
AU  - Davidović, Vesna
AU  - Mekić, Cvijan
AU  - Dimitrijević, Blagoje
AU  - Cincović, Marko
AU  - Stanković, Branislav
PY  - 2020
UR  - http://r.istocar.bg.ac.rs/handle/123456789/709
AB  - The investigation was performed on two groups of primiparous and multiparous healthy dewormed Alpine dairy goats (25 each) during peripartal period. Blood samples were collected (jugular venipuncture) 10-15 days before and 10-15 and 30 days after the parturition into BD SST-II Advance (3.5 mL) and BD NaF 3.0 mg Na2EDTA 6.0 mg (2 mL) vacutainers, cooled and
centrifuged (1500 r/min, 15 minutes and ≤1300 r/min, 10 minutes, respectively). Glucose, non-esterified fatty acids (NEFA) and β-hydroxybutyrate (BHBA) concentrations in blood sera were determined using A15 automatic spectrophotometric analyzer (Biosystem, Spain). Simultaneously, body condition scoring (BCS) was performed by Villaquiran et al. (2007) method. The obtained data were analyzed by IBM SPSS statistics 21. The glucose concentration inclined to increase in both groups. Differences between glucose levels were significant (P<0.05) 15 days before and 15 days after, as well as 15 and 30 days after the parturition, and very significant (P<0.01) 15 days before and 30 days after the parturition. The BHBA blood levels significantly differed 15 days before and 30 days after and 15 and 30 days after the parturition (P<0.05). BHBA concentration peaked at week 2 postpartum, following the increase of NEFA, providing the substrate for BHBA synthesis. NEFA levels significantly (P<0.05) differed 15 days before and 15 days after the parturition. Goats’ BCS ranged from 2 to 4 and significantly depended on glucose (r=0.392; P<0.05) and BHBA (r=0.317; P<0.05) level 15 days before parturition. BCS 30 days postpartum very significantly depended on the glucose level (r=0.450; P<0.01), significantly higher than the concentration of BHBA (r=0.351; P<0.05) and NEFA concentration (r=-0.304; P<0.05). BCS 15 days before parturition did not depend on the NEFA concentration. Fifteen days after the parturition BCS did not statistically depend on the observed indicators. Obtained data suggest that knowledge of BCS and energy indicators levels may be very useful in research and practice in order to appreciate energy metabolism of pregnant and lactating dairy ruminants, particularly dairy goats. These data are poorly documented for goats, but they can reveal early pathological metabolic changes in transiting female goat organism, enabling successful prophylactic, as well as, therapeutic intervention.
PB  - SIVAR (Italian Society of Farm Animals Veterinary Practitioners)
T2  - Large Animal Review
T1  - Energy metabolism indicators  and body condition in peripartal period of Alpine goats
VL  - 26
IS  - 1
SP  - 13
EP  - 18
UR  - https://hdl.handle.net/21.15107/rcub_ristocar_709
ER  - 

@article{
author = "Milošević-Stanković, Ivana and Hristov, Slavča and Maksimović, Nevena and Popović, Blaženka and Davidović, Vesna and Mekić, Cvijan and Dimitrijević, Blagoje and Cincović, Marko and Stanković, Branislav",
year = "2020",
abstract = "The investigation was performed on two groups of primiparous and multiparous healthy dewormed Alpine dairy goats (25 each) during peripartal period. Blood samples were collected (jugular venipuncture) 10-15 days before and 10-15 and 30 days after the parturition into BD SST-II Advance (3.5 mL) and BD NaF 3.0 mg Na2EDTA 6.0 mg (2 mL) vacutainers, cooled and
centrifuged (1500 r/min, 15 minutes and ≤1300 r/min, 10 minutes, respectively). Glucose, non-esterified fatty acids (NEFA) and β-hydroxybutyrate (BHBA) concentrations in blood sera were determined using A15 automatic spectrophotometric analyzer (Biosystem, Spain). Simultaneously, body condition scoring (BCS) was performed by Villaquiran et al. (2007) method. The obtained data were analyzed by IBM SPSS statistics 21. The glucose concentration inclined to increase in both groups. Differences between glucose levels were significant (P<0.05) 15 days before and 15 days after, as well as 15 and 30 days after the parturition, and very significant (P<0.01) 15 days before and 30 days after the parturition. The BHBA blood levels significantly differed 15 days before and 30 days after and 15 and 30 days after the parturition (P<0.05). BHBA concentration peaked at week 2 postpartum, following the increase of NEFA, providing the substrate for BHBA synthesis. NEFA levels significantly (P<0.05) differed 15 days before and 15 days after the parturition. Goats’ BCS ranged from 2 to 4 and significantly depended on glucose (r=0.392; P<0.05) and BHBA (r=0.317; P<0.05) level 15 days before parturition. BCS 30 days postpartum very significantly depended on the glucose level (r=0.450; P<0.01), significantly higher than the concentration of BHBA (r=0.351; P<0.05) and NEFA concentration (r=-0.304; P<0.05). BCS 15 days before parturition did not depend on the NEFA concentration. Fifteen days after the parturition BCS did not statistically depend on the observed indicators. Obtained data suggest that knowledge of BCS and energy indicators levels may be very useful in research and practice in order to appreciate energy metabolism of pregnant and lactating dairy ruminants, particularly dairy goats. These data are poorly documented for goats, but they can reveal early pathological metabolic changes in transiting female goat organism, enabling successful prophylactic, as well as, therapeutic intervention.",
publisher = "SIVAR (Italian Society of Farm Animals Veterinary Practitioners)",
journal = "Large Animal Review",
title = "Energy metabolism indicators  and body condition in peripartal period of Alpine goats",
volume = "26",
number = "1",
pages = "13-18",
url = "https://hdl.handle.net/21.15107/rcub_ristocar_709"
}

Milošević-Stanković, I., Hristov, S., Maksimović, N., Popović, B., Davidović, V., Mekić, C., Dimitrijević, B., Cincović, M.,& Stanković, B.. (2020). Energy metabolism indicators  and body condition in peripartal period of Alpine goats. in Large Animal Review
SIVAR (Italian Society of Farm Animals Veterinary Practitioners)., 26(1), 13-18.
https://hdl.handle.net/21.15107/rcub_ristocar_709

Milošević-Stanković I, Hristov S, Maksimović N, Popović B, Davidović V, Mekić C, Dimitrijević B, Cincović M, Stanković B. Energy metabolism indicators  and body condition in peripartal period of Alpine goats. in Large Animal Review. 2020;26(1):13-18.
https://hdl.handle.net/21.15107/rcub_ristocar_709 .

Milošević-Stanković, Ivana, Hristov, Slavča, Maksimović, Nevena, Popović, Blaženka, Davidović, Vesna, Mekić, Cvijan, Dimitrijević, Blagoje, Cincović, Marko, Stanković, Branislav, "Energy metabolism indicators  and body condition in peripartal period of Alpine goats" in Large Animal Review, 26, no. 1 (2020):13-18,
https://hdl.handle.net/21.15107/rcub_ristocar_709 .

TY  - JOUR
AU  - Dimitrijević, Blagoje
AU  - Jović, Slavoljub
AU  - Ostojić Andrić, Dušica
AU  - Savić, Mila
AU  - Bečkei, Žolt
AU  - Davidović, Vesna
AU  - Joksimović-Todorović, Mirjana
PY  - 2016
UR  - http://r.istocar.bg.ac.rs/handle/123456789/490
AB  - The aim of this study was to determine and evaluate the basic haematological parameters in conditions of natural infection of sheep with Strongyloides papillosus, as well as after the administration of antihelminthic albendazole (ABZ). Based on the intensity of infection with S. papillosus the sheep were divided into three groups: mild, moderate and high, and after that the sheep received a single dose of ABZ of 5mg/kg per body weight, per orally. Sampling of faeces and blood for parasitological and haematological assaying respectively, was performed on the 0 and the 21st day after the treatment with ABZ. The presence of parasitic infection with S. papillosus leads to a decrease of erythrocyte count, while the lowest values were established in the group with the highest intensity of parasitic infection (p ˂ 0.001). After treatment with ABZ the decrease of erythrocyte count was more prominent, which was, based on comparison with control groups C1 and C2, unequivocally established to be the consequence of treatment with ABZ. Detected values of haematocrit and erythrocyte indices indicated the presence of parasitic infection: the lowest values were established in the group with the highest intensity of parasitic infection. After treatment with ABZ haematocrit levels in control group C2 were statistically significantly lower compared to the control group C1 (p ˂ 0.001). In the presence of parasitic infection, the neutrophil and eosinophil counts increased almost linearly up to the value of 44.24±2.50% and 13.29±0.61% respectively, in the group of sheep with the highest intensity of parasitic infection (p ˂ 0.001; compared to control group C1). After treatment with ABZ the decrease of the number of these white blood cells is statistically significant (p ˂ 0.001). Bearing in mind our previous research and the connection of disbalanced redox equilibrium after the treatment with ABZ with changes, it is necessary to include antioxidative substances in the anti-parasitic treatment protocols.
AB  - Cilj ovog istraživanja bio je da se utvrde i procene osnovni hematološki parametri u uslovima prirodne infekcije ovaca sa Strongyloides papillosus, kao i nakon primene antihelmintika albendazola (ABZ). Na osnovu intenziteta infekcije sa S. papillosus ovce su podeljene u tri grupe: niski, srednji i visoki intenzitet infekcije, a zatim su ovce jednokratno dobile peroralno ABZ, u terapijskoj dozi od 5 mg/kg telesne mase. Uzorkovanje fecesa za parazitološka i za hematološka ispitivanja obavljeno je nultog i 21. dana od primene ABZ. Utvrđeno je da prisustvo parazitske infekcije sa S. papillosus dovodi do pada broja eritrocita, pri čemu su najniže vrednosti utvrđene u grupi sa najvećim intenzitetom parazitske infekcije (p ˂ 0,001). Nakon terapije sa ABZ pad broja eritrocita je izraženiji, što je nesumnjivo nastalo kao posledica terapije ABZ (na osnovu poređenja C1 i C2). Utvrđene vrednosti hematokrita i eritrocitnih indeksa su ukazivali na postojanje parazitske infekcije; najniže vrednosti su utvrđene kod grupe sa najvećim intenzitetom parazitske infekcije. Nakon terapije sa ABZ vrednosti hematokrita kod C2 bile su statistički značajno niže u odnosu na kontrolnu grupu C1 (p ˂ 0,001). U prisustvu parazitske infekcije broj neutrofila i eozinofila povećava se gotovo linearno, do vrednosti od 44,24±2,50% kod neutrofila, odnosno od 13,29±0,61% kod eozinofila u grupi ovaca sa najvećim intenzitetom parazitske infekcije (p ˂ 0,001). Nakon terapije sa ABZ broj ovih ćelija bele krvne loze smanjuje se statistički značajno (p ˂ 0,001). Imajući u vidu naša prethodna istraživanja i povezanost narušene redoks ravnoteže posle terapije sa ABZ sa promenama utvrđenim u ovom istraživanju, neophodno je u antiparazitske terapijske protokole uključiti antioksidativne supstance.
PB  - Institute for Animal Husbandry, Belgrade
T2  - Biotechnology in Animal Husbandry
T1  - Infection with Strongyloides papillosus in sheep: Effect of parasitic infection and treatment with albendazole on basic haematological parameters
T1  - Infekcija ovaca sa Strongyloides papillosus - uticaj intenziteta parazitske infekcije i terapije albendazolom na vrednosti osnovnih hematoloških parametara
VL  - 32
IS  - 4
SP  - 369
EP  - 381
DO  - 10.2298/bah1604369D
ER  - 

@article{
author = "Dimitrijević, Blagoje and Jović, Slavoljub and Ostojić Andrić, Dušica and Savić, Mila and Bečkei, Žolt and Davidović, Vesna and Joksimović-Todorović, Mirjana",
year = "2016",
abstract = "The aim of this study was to determine and evaluate the basic haematological parameters in conditions of natural infection of sheep with Strongyloides papillosus, as well as after the administration of antihelminthic albendazole (ABZ). Based on the intensity of infection with S. papillosus the sheep were divided into three groups: mild, moderate and high, and after that the sheep received a single dose of ABZ of 5mg/kg per body weight, per orally. Sampling of faeces and blood for parasitological and haematological assaying respectively, was performed on the 0 and the 21st day after the treatment with ABZ. The presence of parasitic infection with S. papillosus leads to a decrease of erythrocyte count, while the lowest values were established in the group with the highest intensity of parasitic infection (p ˂ 0.001). After treatment with ABZ the decrease of erythrocyte count was more prominent, which was, based on comparison with control groups C1 and C2, unequivocally established to be the consequence of treatment with ABZ. Detected values of haematocrit and erythrocyte indices indicated the presence of parasitic infection: the lowest values were established in the group with the highest intensity of parasitic infection. After treatment with ABZ haematocrit levels in control group C2 were statistically significantly lower compared to the control group C1 (p ˂ 0.001). In the presence of parasitic infection, the neutrophil and eosinophil counts increased almost linearly up to the value of 44.24±2.50% and 13.29±0.61% respectively, in the group of sheep with the highest intensity of parasitic infection (p ˂ 0.001; compared to control group C1). After treatment with ABZ the decrease of the number of these white blood cells is statistically significant (p ˂ 0.001). Bearing in mind our previous research and the connection of disbalanced redox equilibrium after the treatment with ABZ with changes, it is necessary to include antioxidative substances in the anti-parasitic treatment protocols., Cilj ovog istraživanja bio je da se utvrde i procene osnovni hematološki parametri u uslovima prirodne infekcije ovaca sa Strongyloides papillosus, kao i nakon primene antihelmintika albendazola (ABZ). Na osnovu intenziteta infekcije sa S. papillosus ovce su podeljene u tri grupe: niski, srednji i visoki intenzitet infekcije, a zatim su ovce jednokratno dobile peroralno ABZ, u terapijskoj dozi od 5 mg/kg telesne mase. Uzorkovanje fecesa za parazitološka i za hematološka ispitivanja obavljeno je nultog i 21. dana od primene ABZ. Utvrđeno je da prisustvo parazitske infekcije sa S. papillosus dovodi do pada broja eritrocita, pri čemu su najniže vrednosti utvrđene u grupi sa najvećim intenzitetom parazitske infekcije (p ˂ 0,001). Nakon terapije sa ABZ pad broja eritrocita je izraženiji, što je nesumnjivo nastalo kao posledica terapije ABZ (na osnovu poređenja C1 i C2). Utvrđene vrednosti hematokrita i eritrocitnih indeksa su ukazivali na postojanje parazitske infekcije; najniže vrednosti su utvrđene kod grupe sa najvećim intenzitetom parazitske infekcije. Nakon terapije sa ABZ vrednosti hematokrita kod C2 bile su statistički značajno niže u odnosu na kontrolnu grupu C1 (p ˂ 0,001). U prisustvu parazitske infekcije broj neutrofila i eozinofila povećava se gotovo linearno, do vrednosti od 44,24±2,50% kod neutrofila, odnosno od 13,29±0,61% kod eozinofila u grupi ovaca sa najvećim intenzitetom parazitske infekcije (p ˂ 0,001). Nakon terapije sa ABZ broj ovih ćelija bele krvne loze smanjuje se statistički značajno (p ˂ 0,001). Imajući u vidu naša prethodna istraživanja i povezanost narušene redoks ravnoteže posle terapije sa ABZ sa promenama utvrđenim u ovom istraživanju, neophodno je u antiparazitske terapijske protokole uključiti antioksidativne supstance.",
publisher = "Institute for Animal Husbandry, Belgrade",
journal = "Biotechnology in Animal Husbandry",
title = "Infection with Strongyloides papillosus in sheep: Effect of parasitic infection and treatment with albendazole on basic haematological parameters, Infekcija ovaca sa Strongyloides papillosus - uticaj intenziteta parazitske infekcije i terapije albendazolom na vrednosti osnovnih hematoloških parametara",
volume = "32",
number = "4",
pages = "369-381",
doi = "10.2298/bah1604369D"
}

Dimitrijević, B., Jović, S., Ostojić Andrić, D., Savić, M., Bečkei, Ž., Davidović, V.,& Joksimović-Todorović, M.. (2016). Infection with Strongyloides papillosus in sheep: Effect of parasitic infection and treatment with albendazole on basic haematological parameters. in Biotechnology in Animal Husbandry
Institute for Animal Husbandry, Belgrade., 32(4), 369-381.
https://doi.org/10.2298/bah1604369D

Dimitrijević B, Jović S, Ostojić Andrić D, Savić M, Bečkei Ž, Davidović V, Joksimović-Todorović M. Infection with Strongyloides papillosus in sheep: Effect of parasitic infection and treatment with albendazole on basic haematological parameters. in Biotechnology in Animal Husbandry. 2016;32(4):369-381.
doi:10.2298/bah1604369D .

Dimitrijević, Blagoje, Jović, Slavoljub, Ostojić Andrić, Dušica, Savić, Mila, Bečkei, Žolt, Davidović, Vesna, Joksimović-Todorović, Mirjana, "Infection with Strongyloides papillosus in sheep: Effect of parasitic infection and treatment with albendazole on basic haematological parameters" in Biotechnology in Animal Husbandry, 32, no. 4 (2016):369-381,
https://doi.org/10.2298/bah1604369D . .